Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 75,447 A→G C12R (TGC→CGC)  thiB ← thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091375,4470AG100.0% 77.6 / NA 23C12R (TGC→CGC) thiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

CCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAT  >  NC_000913/75315‑75552
                                                                                                                                    |                                                                                                         
ccaccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaa                                                                                                     >  1:1100148/1‑139 (MQ=255)
  accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaaca                                                                                                   >  2:272281/1‑139 (MQ=255)
    caGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaacagc                                                                                                 <  2:241876/139‑1 (MQ=255)
              tCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGNGCAGAc                                                                                       >  2:970216/1‑139 (MQ=255)
                       cAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTa                                                                              <  2:1100148/139‑1 (MQ=255)
                           cGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTATGCGCAGCAACAGCAGGGGCAGACATTTTTTTAacac                                                                          >  2:121151/1‑139 (MQ=255)
                                  aaaGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCAc                                                                   >  1:565775/1‑139 (MQ=255)
                                   aaGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTATGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCAcc                                                                  <  1:121151/139‑1 (MQ=255)
                                       cTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaa                                                              >  2:257409/1‑139 (MQ=255)
                                                        ccAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGa                                             <  2:565775/139‑1 (MQ=255)
                                                           ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAgggg                                                                                            <  2:977478/89‑1 (MQ=255)
                                                           ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAgggg                                                                                            >  1:977478/1‑89 (MQ=255)
                                                                ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                     <  1:848428/91‑1 (MQ=255)
                                                                ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                     >  2:848428/1‑91 (MQ=255)
                                                                ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGaa                                     >  1:857370/1‑139 (MQ=255)
                                                                      ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                             >  1:755671/1‑93 (MQ=255)
                                                                      ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                             <  2:755671/93‑1 (MQ=255)
                                                                       gcggcgAAGGAATCGCAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCt                              >  2:244576/1‑139 (MQ=255)
                                                                                             aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                              <  2:669149/101‑1 (MQ=255)
                                                                                             aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                              >  1:669149/1‑101 (MQ=255)
                                                                                             aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCAGCGTTAt        <  1:528868/139‑1 (MQ=255)
                                                                                                   tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                    <  1:1096055/73‑1 (MQ=255)
                                                                                                   tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                    >  2:1096055/1‑73 (MQ=255)
                                                                                                   tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt  >  2:904116/1‑139 (MQ=255)
                                                                                                    cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagc                                                                                                       >  1:154992/1‑37 (MQ=37)
                                                                                                    cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagc                                                                                                       <  2:154992/37‑1 (MQ=37)
                                                                                                    cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGTAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                             <  1:758458/63‑1 (MQ=255)
                                                                                                    cAGAACGGGTTTAGCCAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                             >  2:758458/1‑63 (MQ=255)
                                                                                                                                    |                                                                                                         
CCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAT  >  NC_000913/75315‑75552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: