Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,148,691 G→A *357* (TAG→TAA plsX → putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,148,6910GA100.0% 103.0 / NA 32*357* (TAG→TAAplsXputative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

GGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC  >  NC_000913/1148562‑1148807
                                                                                                                                 |                                                                                                                    
ggTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCt                                                                                                             <  1:356900/139‑1 (MQ=255)
    gcgATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCa                                                                                                         >  1:764891/1‑139 (MQ=255)
                    tGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTg                                                                                         >  2:1002934/1‑139 (MQ=255)
                           ggTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                  <  2:535355/139‑1 (MQ=255)
                              gCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                  >  2:820657/1‑136 (MQ=255)
                              gCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                  <  1:820657/136‑1 (MQ=255)
                              gCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACgg                                                                               >  1:515935/1‑139 (MQ=255)
                                           ccTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGc                                                                                        <  1:400563/117‑1 (MQ=255)
                                           ccTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGc                                                                                        >  2:400563/1‑117 (MQ=255)
                                            cTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGaaaa                                                                 >  1:784537/1‑139 (MQ=255)
                                                   aaTTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTg                                                          <  2:784537/139‑1 (MQ=255)
                                                       gCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                      <  1:328116/139‑1 (MQ=255)
                                                       gCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                      <  2:374255/139‑1 (MQ=255)
                                                         cGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                    <  2:58937/139‑1 (MQ=255)
                                                                          tataCCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGatta                                   <  2:835770/139‑1 (MQ=255)
                                                                               ccAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACa                                                  >  1:194075/1‑119 (MQ=255)
                                                                               ccAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACa                                                  <  2:194075/119‑1 (MQ=255)
                                                                                 aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTAc                            >  2:343068/1‑139 (MQ=255)
                                                                                  gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGa                                                                                                                 >  1:280703/1‑53 (MQ=255)
                                                                                  gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGa                                                                                                                 <  2:280703/53‑1 (MQ=255)
                                                                                  gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCa                                                                                              <  2:410104/72‑1 (MQ=255)
                                                                                  gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCa                                                                                              >  1:410104/1‑72 (MQ=255)
                                                                                   cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                     >  1:161432/1‑80 (MQ=255)
                                                                                   cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                     <  2:161432/80‑1 (MQ=255)
                                                                                      gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTg                                <  2:304196/130‑1 (MQ=255)
                                                                                      gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTg                                >  1:304196/1‑130 (MQ=255)
                                                                                                          ggCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTg   <  1:343068/139‑1 (MQ=255)
                                                                                                           gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCg                                                                                                      >  2:1074543/1‑39 (MQ=38)
                                                                                                           gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCg                                                                                                      <  1:1074543/39‑1 (MQ=255)
                                                                                                           gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa         >  2:839539/1‑132 (MQ=255)
                                                                                                           gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa         <  1:839539/132‑1 (MQ=255)
                                                                                                           gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc  >  2:1043654/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                    
GGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC  >  NC_000913/1148562‑1148807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: