Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,693	C→T	P32L (CCG→CTG)	ycfJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,693	0	C	T	100.0%	155.8 / NA	48	P32L (CCG→CTG)	ycfJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/21);  total (27/21)

CGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGT  >  NC_000913/1167557‑1167818                                                                                                                                         |                                                                                                                              cGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACCGGGCCTGc                                                                                                                             >  2:964196/1‑139 (MQ=255)  gCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTGCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCa                                                                                                                            <  2:144708/139‑1 (MQ=255)       cATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACg                                                                                                                       <  1:391159/139‑1 (MQ=255)         tGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     <  2:101826/139‑1 (MQ=255)            ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAg                                                                                                                  >  2:1037861/1‑139 (MQ=255)             tGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                 >  1:1078268/1‑139 (MQ=255)              ggCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgt                                                                                                                >  2:173894/1‑139 (MQ=255)                 aTAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                             >  1:888705/1‑139 (MQ=255)                     cTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCt                                                                                                         >  2:748004/1‑139 (MQ=255)                        cAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                           <  2:970435/118‑1 (MQ=255)                        cAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                           >  1:970435/1‑118 (MQ=255)                         aaaGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACCGGGCCTGCAATACGCTCAGGTTGTTTCTGCaa                                                                                                     <  1:964196/139‑1 (MQ=255)                           agaagGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  1:173894/139‑1 (MQ=255)                               ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     >  1:671443/1‑117 (MQ=255)                               ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGCATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     <  2:671443/117‑1 (MQ=255)                                gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                              >  1:133935/1‑139 (MQ=255)                                         ccGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:713288/139‑1 (MQ=255)                                                tAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTaaa                                                                              >  1:29754/1‑139 (MQ=255)                                                         gTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGt                                                                     >  1:970043/1‑139 (MQ=255)                                                           tGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     >  1:452784/1‑121 (MQ=255)                                                           tGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:452784/121‑1 (MQ=255)                                                             gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAgg                                                                 >  2:440953/1‑139 (MQ=255)                                                             gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACACCAATCAAGGAAACGGTTAAAACACCGCGTCAgg                                                                 >  1:728397/1‑139 (MQ=255)                                                              cGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGa                                                                >  1:285100/1‑139 (MQ=255)                                                                   aTCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTc                                                           >  2:314088/1‑139 (MQ=255)                                                                    tCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCg                                                          >  2:389814/1‑139 (MQ=255)                                                                            ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccc                                                                                                  <  1:1090251/91‑1 (MQ=255)                                                                            ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccc                                                                                                  >  2:1090251/1‑91 (MQ=255)                                                                            ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                  <  1:748004/139‑1 (MQ=255)                                                                              tgtCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                >  1:515504/1‑139 (MQ=255)                                                                              tgtCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                >  2:1023508/1‑139 (MQ=255)                                                                                  gCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:420532/98‑1 (MQ=255)                                                                                  gCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     >  1:420532/1‑98 (MQ=255)                                                                                       tGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCAt                                       >  2:135127/1‑139 (MQ=255)                                                                                              ggCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCCATCAAGGaa                                                                                       <  1:761533/84‑1 (MQ=255)                                                                                              ggCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                       >  2:761533/1‑84 (MQ=255)                                                                                              ggCGTAGCGGCAGTGGCCAGGCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGAc                                <  1:389814/139‑1 (MQ=255)                                                                                                cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATa                                                                                                                         <  2:516145/48‑1 (MQ=255)                                                                                                cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATa                                                                                                                         >  1:516145/1‑48 (MQ=255)                                                                                                cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCg                              >  2:838182/1‑139 (MQ=255)                                                                                                   aGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  2:175827/67‑1 (MQ=255)                                                                                                   aGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   >  1:175827/1‑67 (MQ=255)                                                                                                            ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                  <  2:888705/139‑1 (MQ=255)                                                                                                            ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                  <  2:970043/139‑1 (MQ=255)                                                                                                                 gTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGCAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCg             <  2:728397/139‑1 (MQ=255)                                                                                                                            tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   >  1:488410/1‑42 (MQ=255)                                                                                                                            tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  2:488410/42‑1 (MQ=255)                                                                                                                            tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGt  <  1:1023508/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                              CGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGT  >  NC_000913/1167557‑1167818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑