Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,263,255 A→G R82R (CGT→CGC lolB ← lipoprotein localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,263,2550AG100.0% 110.8 / NA 32R82R (CGT→CGClolBlipoprotein localization factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

GGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGAT  >  NC_000913/1263123‑1263376
                                                                                                                                    |                                                                                                                         
ggTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     >  2:1025266/1‑139 (MQ=255)
ggTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     >  1:463981/1‑139 (MQ=255)
                             cATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTtgctg                                                                                                 <  2:1021730/130‑1 (MQ=255)
                             cATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTtgctg                                                                                                 >  1:1021730/1‑130 (MQ=255)
                              aTCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAg                                                                                       <  2:463981/139‑1 (MQ=255)
                                     gTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGt                                                                                <  1:1020067/139‑1 (MQ=255)
                                             cTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACtttt                                                                        >  1:1023091/1‑139 (MQ=255)
                                                     tATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     <  1:57912/86‑1 (MQ=255)
                                                     tATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     >  2:57912/1‑86 (MQ=255)
                                                            gACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTttg                                                                      >  1:1004334/1‑126 (MQ=255)
                                                            gACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTttg                                                                      <  2:1004334/126‑1 (MQ=255)
                                                                            cccGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGcgccgc                                         <  1:1021003/139‑1 (MQ=255)
                                                                              cGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGa                                       <  1:1025266/139‑1 (MQ=255)
                                                                                    aGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGa                                 <  1:249830/139‑1 (MQ=255)
                                                                                    aGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGa                                 >  2:249830/1‑139 (MQ=255)
                                                                                    aGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGa                                 <  2:1023091/139‑1 (MQ=255)
                                                                                      cATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGaa                                                 <  1:79445/121‑1 (MQ=255)
                                                                                      cATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGaa                                                 >  2:79445/1‑121 (MQ=255)
                                                                                      cATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCCCCGCGAGTCTGATAc                              >  2:518665/1‑139 (MQ=255)
                                                                                           aGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTc                                    <  1:100523/129‑1 (MQ=255)
                                                                                           aGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTc                                    >  2:100523/1‑129 (MQ=255)
                                                                                                       gTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     >  1:889253/1‑36 (MQ=255)
                                                                                                       gTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAg                                                                                                                     <  2:889253/36‑1 (MQ=255)
                                                                                                       gTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAg                                                                                       >  2:1079558/1‑66 (MQ=255)
                                                                                                       gTGCTGCCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAg                                                                                       <  1:1079558/66‑1 (MQ=255)
                                                                                                        tgctgcCCAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCAc             >  2:366047/1‑139 (MQ=255)
                                                                                                             cccAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGt                                                                 <  1:943333/82‑1 (MQ=255)
                                                                                                             cccAATGGGTTAGTGAGCAGCAGGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGt                                                                 >  2:943333/1‑82 (MQ=255)
                                                                                                                              cagcagGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTtgctgat  <  2:344119/128‑1 (MQ=255)
                                                                                                                              cagcagGCGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTtgctgat  >  1:344119/1‑128 (MQ=255)
                                                                                                                                gcagGCGGTAGCGATCCTGGCCGGGTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGACATATAAGCg                                                   <  1:767344/77‑1 (MQ=255)
                                                                                                                                gcagGCGGTAGCGATCCTGGCCGGATTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCg                                                   >  2:767344/1‑77 (MQ=255)
                                                                                                                                    |                                                                                                                         
GGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGAT  >  NC_000913/1263123‑1263376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: