Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,276	G→A	G11E (GGA→GAA)	rssA →	putative patatin‑like family phospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,276	0	G	A	100.0%	206.6 / NA	63	G11E (GGA→GAA)	rssA	putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/30);  total (33/30)

CCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGA  >  NC_000913/1289143‑1289402                                                                                                                                      |                                                                                                                               ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTg                                                                                                                           >  1:299647/1‑139 (MQ=255)    ggAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGgcg                                                                                                                        <  2:299647/139‑1 (MQ=255)           aTAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCgag                                                                                                                 >  1:1032449/1‑139 (MQ=255)           aTAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCgag                                                                                                                 >  2:336463/1‑139 (MQ=255)           aTAACGGCGTATTAACCGCCTGAGAAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAAAAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCgag                                                                                                                 <  1:633111/139‑1 (MQ=255)               cGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGt                                                                                                             >  1:575934/1‑139 (MQ=255)                             ccTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                                                                               <  1:336463/139‑1 (MQ=255)                                        cTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGGTAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGt                                                                                            <  1:386434/131‑1 (MQ=255)                                        cTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGt                                                                                            >  2:386434/1‑131 (MQ=255)                                        cTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATg                                                                                    >  2:782457/1‑139 (MQ=255)                                         tATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   <  1:367825/139‑1 (MQ=255)                                           tGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGgcg                                                                                                                        <  2:797732/100‑1 (MQ=255)                                           tGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGgcg                                                                                                                        >  1:797732/1‑100 (MQ=255)                                                   gAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   <  2:983188/129‑1 (MQ=255)                                                   gAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   >  1:983188/1‑129 (MQ=255)                                                     gCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt                                                                       >  2:369040/1‑139 (MQ=255)                                                         tAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGt                                                                   >  1:1051192/1‑139 (MQ=255)                                                          aGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTa                                                                  <  2:1051192/139‑1 (MQ=255)                                                            cGATGTGGCTACGATTGCATTGCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTAtt                                                                <  2:1032449/139‑1 (MQ=255)                                                            cGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTAtt                                                                <  2:792655/139‑1 (MQ=255)                                                              aTGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt                                                                       >  1:659869/1‑130 (MQ=255)                                                              aTGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt                                                                       <  2:659869/130‑1 (MQ=255)                                                              aTGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGa                                                              >  2:89485/1‑139 (MQ=255)                                                                   gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTc                                                                                                        <  1:1079248/92‑1 (MQ=255)                                                                   gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTc                                                                                                        >  2:1079248/1‑92 (MQ=255)                                                                   gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   >  2:954596/1‑113 (MQ=255)                                                                   gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   <  1:954596/113‑1 (MQ=255)                                                                       cGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGatat                                                     <  1:782457/139‑1 (MQ=255)                                                                         aTTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCata                                                                                                   <  2:292797/91‑1 (MQ=255)                                                                         aTTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCata                                                                                                   >  1:292797/1‑91 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGt                                                                                                         <  2:365871/81‑1 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGt                                                                                                         >  1:365871/1‑81 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   <  2:534973/103‑1 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgc                                                                                   >  1:534973/1‑103 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt                                                                       <  2:447547/115‑1 (MQ=255)                                                                             cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt                                                                       >  1:447547/1‑115 (MQ=255)                                                                                       gAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCa                                     >  2:45756/1‑139 (MQ=255)                                                                                          tCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGata                                                      <  2:587551/119‑1 (MQ=255)                                                                                          tCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGata                                                      >  1:587551/1‑119 (MQ=255)                                                                                            ttGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGgc                                                                                                                         >  2:152477/1‑50 (MQ=255)                                                                                            ttGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGgc                                                                                                                         <  1:152477/50‑1 (MQ=255)                                                                                            ttGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCgag                                                                                                                 >  1:862864/1‑58 (MQ=255)                                                                                            ttGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCgag                                                                                                                 <  2:862864/58‑1 (MQ=255)                                                                                                gggAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCga                                                                                                                  >  2:523689/1‑53 (MQ=255)                                                                                                gggAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCga                                                                                                                  <  1:523689/53‑1 (MQ=255)                                                                                                        tGAGAAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTggg                    >  1:277114/1‑139 (MQ=255)                                                                                                          agaAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                  <  1:369040/139‑1 (MQ=255)                                                                                                          agaAAGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                  <  1:89485/139‑1 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGt                                                                                                             <  1:728387/46‑1 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGt                                                                                                             >  2:728387/1‑46 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                                                                               <  2:1073125/60‑1 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                                                                               >  1:1073125/1‑60 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCtggtg                      <  1:1020230/133‑1 (MQ=255)                                                                                                            aaaGATAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCtggtg                      >  2:1020230/1‑133 (MQ=255)                                                                                                                aTAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                                                                               <  2:204271/56‑1 (MQ=255)                                                                                                                aTAAAAATAGGGCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                                                                               >  1:204271/1‑56 (MQ=255)                                                                                                                         gggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcg   >  1:603447/1‑139 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCata                                                                                                   <  2:710337/42‑1 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCata                                                                                                   >  1:710337/1‑42 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaaa                                                                         <  2:789446/68‑1 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaaa                                                                         >  1:789446/1‑68 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcga  >  1:979808/1‑139 (MQ=255)                                                                                                                          ggCTGGCGCTGGAATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcga  >  1:988624/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                               CCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGA  >  NC_000913/1289143‑1289402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑