Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,309,016 C→T *418* (TAG→TAA)  kch ← voltage‑gated potassium channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,309,0160CT100.0% 196.3 / NA 60*418* (TAG→TAA) kchvoltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/28);  total (32/28)

ATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAAC  >  NC_000913/1308884‑1309144
                                                                                                                                    |                                                                                                                                
atGCGTATCTACAGCATGAGGTTGTGCATTCTAAAGCATGAGCGTAACTCGTTATTGTTTCCGATCTACAATATCTAAATGTACTGGGCACCGTTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTAtttt                                                                                                                            <  2:528010/139‑1 (MQ=255)
         cacaGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGAt                                                                                                                   >  1:793297/1‑139 (MQ=255)
                tCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTAc                                                                                                            >  2:517716/1‑139 (MQ=255)
                          cATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCttt                                                                                                  >  1:195470/1‑139 (MQ=255)
                           aTTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                                 <  2:793297/139‑1 (MQ=255)
                                           gTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGt                                                                                 >  1:682883/1‑139 (MQ=255)
                                           gTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGt                                                                                 >  2:1078403/1‑139 (MQ=255)
                                                  gTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCt                                                                          <  1:826679/139‑1 (MQ=255)
                                                      ttGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCACTGaaa                                                                      <  2:195470/139‑1 (MQ=255)
                                                       tGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaaaa                                                                     >  1:466643/1‑139 (MQ=255)
                                                        gTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAAt                                                                    <  2:682883/139‑1 (MQ=255)
                                                                  tACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                          <  2:466643/139‑1 (MQ=255)
                                                                  tACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                          <  1:517716/139‑1 (MQ=255)
                                                                       tatCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                       <  1:521885/73‑1 (MQ=255)
                                                                       tatCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                       >  2:521885/1‑73 (MQ=255)
                                                                          cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                                    >  1:1119192/1‑89 (MQ=255)
                                                                          cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                                    <  2:1119192/89‑1 (MQ=255)
                                                                          cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                                  >  2:237995/1‑139 (MQ=255)
                                                                          cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                                  <  1:237995/139‑1 (MQ=255)
                                                                            aaaTGTACTGGGCACCATTGCGGTGCCCGATAAGGTTACTCTTCCGTCTGTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                >  2:734667/1‑139 (MQ=255)
                                                                            aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                                     >  2:93793/1‑70 (MQ=255)
                                                                            aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                                     <  1:93793/70‑1 (MQ=255)
                                                                            aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                >  2:17899/1‑139 (MQ=255)
                                                                                 tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                            <  1:278565/90‑1 (MQ=255)
                                                                                 tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                            >  2:278565/1‑90 (MQ=255)
                                                                                   cTGGGCACCATTGCGTTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                       <  2:802225/61‑1 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                       >  1:802225/1‑61 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                            <  2:34750/88‑1 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTc                                                                                            >  1:34750/1‑88 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            >  1:674308/1‑104 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            <  2:674308/104‑1 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                               >  2:272187/1‑117 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                               <  1:272187/117‑1 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                                         >  2:586499/1‑139 (MQ=255)
                                                                                   cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                                         >  2:168318/1‑139 (MQ=255)
                                                                                     gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                                       <  1:586499/139‑1 (MQ=255)
                                                                                      ggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                                         >  1:172295/1‑88 (MQ=255)
                                                                                      ggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                                         <  2:172295/88‑1 (MQ=255)
                                                                                         aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAg                                   <  1:1078403/139‑1 (MQ=255)
                                                                                             ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                            <  2:253015/110‑1 (MQ=255)
                                                                                             ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                            >  1:253015/1‑110 (MQ=255)
                                                                                             ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCata                               >  1:190453/1‑139 (MQ=255)
                                                                                                cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCa                            >  1:83047/1‑139 (MQ=255)
                                                                                                cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCa                            >  2:117904/1‑139 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            >  1:35577/1‑86 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            <  2:35577/86‑1 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                                 <  1:1050021/97‑1 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                                 >  2:1050021/1‑97 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                              <  2:1063020/132‑1 (MQ=255)
                                                                                                     cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                              >  1:1063020/1‑132 (MQ=255)
                                                                                                      ccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta                      <  1:117904/139‑1 (MQ=255)
                                                                                                        aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            <  2:206111/83‑1 (MQ=255)
                                                                                                        aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                            >  1:206111/1‑83 (MQ=255)
                                                                                                                       ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                <  2:18128/96‑1 (MQ=255)
                                                                                                                       ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                                >  1:18128/1‑96 (MQ=255)
                                                                                                                       ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCCGAAAATACCATGACCt                                                          <  2:112171/86‑1 (MQ=255)
                                                                                                                       ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCCGAAAATACCATGACCt                                                          >  1:112171/1‑86 (MQ=255)
                                                                                                                          tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAAc  >  2:52079/1‑139 (MQ=255)
                                                                                                                             ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                             <  2:575144/45‑1 (MQ=255)
                                                                                                                             ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                             >  1:575144/1‑45 (MQ=255)
                                                                                                                                    |                                                                                                                                
ATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAAC  >  NC_000913/1308884‑1309144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑