Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,389,242 C→T Q105* (CAA→TAA)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,389,2420CT100.0% 116.7 / NA 36Q105* (CAA→TAA) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

AGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCG  >  NC_000913/1389138‑1389364
                                                                                                        |                                                                                                                          
agaAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTcc                                                                                          <  1:437016/139‑1 (MQ=255)
    aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                      <  2:340098/139‑1 (MQ=255)
       gCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                   >  1:424310/1‑139 (MQ=255)
                 gaggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCgctgg                                                                                                                   >  2:769625/1‑97 (MQ=255)
                 gaggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCgctgg                                                                                                                   <  1:769625/97‑1 (MQ=255)
                 gaggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTg                                                                         >  2:875054/1‑139 (MQ=255)
                  aggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                        <  2:937776/139‑1 (MQ=255)
                                                 agcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                         <  2:424310/139‑1 (MQ=255)
                                                    aCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATg                                      <  2:878143/139‑1 (MQ=255)
                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                 <  2:992363/87‑1 (MQ=255)
                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                 >  1:992363/1‑87 (MQ=255)
                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                      <  2:820703/114‑1 (MQ=255)
                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                      >  1:820703/1‑114 (MQ=255)
                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGt                             >  2:273626/1‑139 (MQ=255)
                                                                 gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                         >  1:15391/1‑59 (MQ=255)
                                                                 gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                         <  2:15391/59‑1 (MQ=255)
                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                         <  1:500366/54‑1 (MQ=255)
                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                         >  2:500366/1‑54 (MQ=255)
                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAaca                    >  2:420615/1‑139 (MQ=255)
                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCgctgg                                                                                                                   <  1:1061448/39‑1 (MQ=255)
                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCgctgg                                                                                                                   >  2:1061448/1‑39 (MQ=255)
                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                         >  2:84899/1‑113 (MQ=255)
                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                         <  1:84899/113‑1 (MQ=255)
                                                                            gtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCg                                                                                        >  1:461446/1‑65 (MQ=255)
                                                                            gtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCg                                                                                        <  2:461446/65‑1 (MQ=255)
                                                                                gATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcagg          >  1:724445/1‑139 (MQ=255)
                                                                                     ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCg                                                                                                       <  2:936137/41‑1 (MQ=255)
                                                                                     ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCg                                                                                                       >  1:936137/1‑41 (MQ=255)
                                                                                     ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCaggcaggc     >  2:107836/1‑139 (MQ=255)
                                                                                       ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                              >  2:470787/1‑64 (MQ=255)
                                                                                       ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                              <  1:470787/64‑1 (MQ=255)
                                                                                       ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcgc   >  1:286691/1‑139 (MQ=255)
                                                                                        cgcgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg  <  1:420615/139‑1 (MQ=255)
                                                                                        cgcgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg  <  1:107836/139‑1 (MQ=255)
                                                                                          cgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                           >  2:598383/1‑80 (MQ=255)
                                                                                          cgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                           <  1:598383/80‑1 (MQ=255)
                                                                                                        |                                                                                                                          
AGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCG  >  NC_000913/1389138‑1389364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: