Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,798	A→G	L283P (CTA→CCA)	nadC ←	quinolinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,798	0	A	G	100.0%	177.8 / NA	49	L283P (CTA→CCA)	nadC	quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

AAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAT  >  NC_000913/117664‑117919                                                                                                                                       |                                                                                                                          aaaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGgcgc                                                                                                                       >  2:436781/1‑139 (MQ=255)  aaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                      <  2:477522/139‑1 (MQ=255)   aGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCAc                                                                                                                     <  1:472833/139‑1 (MQ=255)       aaGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAAATATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGAc                                                                                                                 >  2:687191/1‑139 (MQ=255)        aGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                <  2:981293/139‑1 (MQ=255)                                aaaGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCg                                                                                        >  2:588689/1‑139 (MQ=255)                                     tGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCaaa                                                                                   <  2:706642/139‑1 (MQ=255)                                      ggggAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAAt                                                                                  >  2:167039/1‑139 (MQ=255)                                       gggAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAAtt                                                                                 >  1:170663/1‑139 (MQ=255)                                          aaGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                  <  1:459333/103‑1 (MQ=255)                                          aaGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                  >  2:459333/1‑103 (MQ=255)                                            ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  2:855564/139‑1 (MQ=255)                                            ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  1:436781/139‑1 (MQ=255)                                            ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  1:167039/139‑1 (MQ=255)                                            ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  1:687191/139‑1 (MQ=255)                                              ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                          <  2:1004467/139‑1 (MQ=255)                                                   ttATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGttt                                                                     <  1:588689/139‑1 (MQ=255)                                                               gATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGt                                                         >  1:959137/1‑139 (MQ=255)                                                                         aTAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAgtgt                                                                       <  1:859159/115‑1 (MQ=255)                                                                         aTAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAgtgt                                                                       >  2:859159/1‑115 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                <  1:561609/65‑1 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                >  2:561609/1‑65 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCa                                                                >  2:123939/1‑113 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCa                                                                <  1:123939/113‑1 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGac                                                 <  1:934576/128‑1 (MQ=255)                                                                                  tCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGac                                                 >  2:934576/1‑128 (MQ=255)                                                                                      cTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  1:843463/97‑1 (MQ=255)                                                                                      cTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            >  2:843463/1‑97 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGt                                                              <  2:630549/105‑1 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGt                                                              >  1:630549/1‑105 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCg                            <  2:959137/139‑1 (MQ=255)                                                                                            gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCg                            >  1:370440/1‑139 (MQ=255)                                                                                                aaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                  <  2:600436/49‑1 (MQ=255)                                                                                                aaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                  >  1:600436/1‑49 (MQ=255)                                                                                                            aGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACgg            >  2:556114/1‑139 (MQ=255)                                                                                                             ggTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAACTTCACGc                                                                            >  2:138423/1‑74 (MQ=255)                                                                                                             ggTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                            <  1:138423/74‑1 (MQ=255)                                                                                                              gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACgg            >  1:425277/1‑137 (MQ=255)                                                                                                              gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACgg            <  2:425277/137‑1 (MQ=255)                                                                                                                  aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAg                                                   <  2:314224/94‑1 (MQ=255)                                                                                                                  aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAg                                                   >  1:314224/1‑94 (MQ=255)                                                                                                                  aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcgc      >  2:684164/1‑139 (MQ=255)                                                                                                                      cTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt  <  1:556114/139‑1 (MQ=255)                                                                                                                              tgtTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg       >  1:287369/1‑126 (MQ=255)                                                                                                                              tgtTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg       <  2:287369/126‑1 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                              <  1:292909/53‑1 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                              >  2:292909/1‑53 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt  <  2:644045/129‑1 (MQ=255)                                                                                                                                tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt  >  1:644045/1‑129 (MQ=255)                                                                                                                                 ttagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt  >  1:209946/1‑128 (MQ=255)                                                                                                                                 ttagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt  <  2:209946/128‑1 (MQ=255)                                                                                                                                       |                                                                                                                          AAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAT  >  NC_000913/117664‑117919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑