| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,459,052 | G→A | *263* (TAG→TAA) | paaG → | 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,459,052 | 0 | G | A | 97.5% | 124.5 / NA | 40 | *263* (TAG→TAA) | paaG | 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/17); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG > NC_000913/1458932‑1459181 | gCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGAGAAATGTGCa > 2:774962/1‑139 (MQ=255) cAACTGGATCTGGGACGTGACTATCAGCGACTTGCCGGACGGAGAGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGGTAAATGTGCAAACTGTGGCa < 2:521103/139‑1 (MQ=255) cAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa > 2:454984/1‑139 (MQ=255) cAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa > 1:771453/1‑139 (MQ=255) tGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGa > 2:91048/1‑139 (MQ=255) gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGAtt > 1:1031809/1‑139 (MQ=255) tGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGa < 2:1031809/139‑1 (MQ=255) tGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGa < 1:91048/139‑1 (MQ=255) cGTGACTATCAGCGACTTGCCGGACGGAGCGCCGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGc < 2:771453/139‑1 (MQ=255) gTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCa > 1:396997/1‑139 (MQ=255) tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc > 1:136600/1‑139 (MQ=255) tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc > 2:88694/1‑139 (MQ=255) cTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt < 2:851516/139‑1 (MQ=255) aTCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATgg > 1:892014/1‑139 (MQ=255) aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACGGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATAGGggcagg > 1:674402/1‑139 (MQ=255) aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTCGCAGTGATTGGGAGCGGCACCATGGGggcagg < 2:674402/139‑1 (MQ=255) cTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggc < 1:481377/139‑1 (MQ=255) gTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg < 1:88694/139‑1 (MQ=255) aGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca < 1:454984/139‑1 (MQ=255) gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATa > 1:853063/1‑60 (MQ=255) gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATa < 2:853063/60‑1 (MQ=255) tgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCa > 1:663398/1‑139 (MQ=255) gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc > 1:914754/1‑123 (MQ=255) gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc < 2:914754/123‑1 (MQ=255) cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATa > 1:433819/1‑51 (MQ=255) cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATa < 2:433819/51‑1 (MQ=255) gTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg > 2:81668/1‑82 (MQ=255) gTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg < 1:81668/82‑1 (MQ=255) aCGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGTAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg > 2:803569/1‑111 (MQ=255) aCGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg < 1:803569/111‑1 (MQ=255) cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg > 2:460991/1‑80 (MQ=255) cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg < 1:460991/80‑1 (MQ=255) cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg < 2:396997/139‑1 (MQ=255) cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg > 2:917587/1‑139 (MQ=255) aCCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa > 2:307212/1‑50 (MQ=255) aCCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa < 1:307212/50‑1 (MQ=255) aCCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGa > 1:948956/1‑139 (MQ=255) ggggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATgggg < 2:643126/62‑1 (MQ=255) ggggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATgggg > 1:643126/1‑62 (MQ=255) ggggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcg > 2:422733/1‑139 (MQ=255) | GCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG > NC_000913/1458932‑1459181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |