Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,144	C→A	L279F (TTG→TTT)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,144	0	C	A	100.0%	198.4 / NA	61	L279F (TTG→TTT)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/32);  total (29/32)

ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGA  >  NC_000913/1525015‑1525278                                                                                                                                  |                                                                                                                                       aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                               >  2:1068106/1‑139 (MQ=255) aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                               <  2:137232/139‑1 (MQ=255) aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                               <  2:222579/139‑1 (MQ=255) aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACAGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                               <  2:244141/139‑1 (MQ=255)      aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg                                                                                                                          >  2:878206/1‑139 (MQ=255)                    agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  2:440485/139‑1 (MQ=255)                    agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  2:466136/139‑1 (MQ=255)                    agGGCAGCCGTCAGCACCACAATGTTCATAATGCTGACGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  1:1068106/139‑1 (MQ=255)                      ggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCg                                                                                                          >  2:935645/1‑139 (MQ=255)                                     cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCa                                                                                           <  1:313118/139‑1 (MQ=255)                                          tGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg                                                                                      <  1:878206/139‑1 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            >  2:971198/1‑112 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  1:971198/112‑1 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                                                                 >  2:503550/1‑139 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                                                                 >  2:87623/1‑139 (MQ=255)                                                 aaTGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATc                                                                               >  2:579847/1‑139 (MQ=255)                                                 aaTGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATc                                                                               >  1:369779/1‑139 (MQ=255)                                                    gctgcCGATATATGGCACACCCAGTTTAGAGAAGAACGTCACGAACGGACTTTGCCCCGCCTGATACGCACTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                                                            >  2:160884/1‑139 (MQ=255)                                                    gctgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                                                            <  2:369779/139‑1 (MQ=255)                                                    gctgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                                                            <  1:1011547/139‑1 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGc                                                                                             >  1:174846/1‑120 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGc                                                                                             <  2:174846/120‑1 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                                                          >  1:2840/1‑139 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                                                          >  2:232743/1‑139 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                                                          >  1:119688/1‑139 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACACCAAATCACACt                                                                          >  2:344594/1‑139 (MQ=255)                                                             atatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                                                                   <  1:1051638/139‑1 (MQ=255)                                                             atatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACACCAAATCACACTGTTAATg                                                                   <  1:344594/139‑1 (MQ=255)                                                                 ggCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg                                                                                                                          >  1:41703/1‑80 (MQ=255)                                                                 ggCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg                                                                                                                          <  2:41703/80‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            >  1:867623/1‑89 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  2:867623/89‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                                                          >  1:576677/1‑139 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACATAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                                                          >  1:613097/1‑139 (MQ=255)                                                                          aGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa                                                      <  1:81900/139‑1 (MQ=255)                                                                          aGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa                                                      <  2:25595/139‑1 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa                                                                     <  1:1128421/122‑1 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa                                                                     >  2:1128421/1‑122 (MQ=255)                                                                                 agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt                                                              >  2:151531/1‑124 (MQ=255)                                                                                 agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt                                                              <  1:151531/124‑1 (MQ=255)                                                                                 agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTg                                               >  1:696545/1‑139 (MQ=255)                                                                                   aaaaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg                                             <  1:935645/139‑1 (MQ=255)                                                                                     aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa                                           <  2:2840/139‑1 (MQ=255)                                                                                     aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa                                           <  1:579847/139‑1 (MQ=255)                                                                                     aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACATAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa                                           <  2:613097/139‑1 (MQ=255)                                                                                                     cTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCgg                           <  2:119688/139‑1 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc                                                       >  1:122484/1‑108 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc                                                       <  2:122484/108‑1 (MQ=255)                                                                                                         gCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            <  2:868193/54‑1 (MQ=255)                                                                                                         gCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                            >  1:868193/1‑54 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa                                                                     <  2:854036/88‑1 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa                                                                     >  1:854036/1‑88 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAc                  >  1:501102/1‑139 (MQ=255)                                                                                                               ccTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAcc                 <  2:696545/139‑1 (MQ=255)                                                                                                                  gATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACccac              <  1:298455/139‑1 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGc                       <  2:1109726/119‑1 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGc                       >  1:1109726/1‑119 (MQ=255)                                                                                                                              aCGGAAAAAACATAACCAGCAACACCACCGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGa  >  2:534062/1‑139 (MQ=255)                                                                                                                               cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg                                                                                      <  1:343662/54‑1 (MQ=255)                                                                                                                               cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg                                                                                      >  2:343662/1‑54 (MQ=255)                                                                                                                               cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                                                                    >  1:708072/1‑56 (MQ=255)                                                                                                                                  |                                                                                                                                       ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGA  >  NC_000913/1525015‑1525278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑