Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,947	T→C	T3A (ACT→GCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,947	0	T	C	100.0%	175.6 / NA	51	T3A (ACT→GCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/23);  total (28/23)

AGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCA  >  NC_000913/150812‑151082                                                                                                                                          |                                                                                                                                        aGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                                                      <  1:5389/139‑1 (MQ=255)  ggTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                                                     >  2:799001/1‑139 (MQ=255)       tCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                                >  1:638264/1‑139 (MQ=255)            gCGGCAGTGGAGGGGACTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTc                                                                                                                           <  2:638264/139‑1 (MQ=255)                 aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      <  2:360242/139‑1 (MQ=255)                        gggCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                        <  2:29200/130‑1 (MQ=255)                        gggCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                        >  1:29200/1‑130 (MQ=255)                                        ccaCTCCTGCATTATTACCCAACAATAAAA‑TTTTATTTGCATTATCCTGAATACAATCAAG‑ATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAAGGTTTCTTTATTGTTATGGTTTgcg                                                                                               <  2:177606/137‑1 (MQ=255)                                                 cATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                               <  2:950266/130‑1 (MQ=255)                                                 cATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                               >  1:950266/1‑130 (MQ=255)                                                 cATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttg                                                                                      >  1:636781/1‑139 (MQ=255)                                                             acaATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                           <  2:636781/139‑1 (MQ=255)                                                                 tAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                                       <  1:184915/139‑1 (MQ=255)                                                                         gTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                         <  1:254918/97‑1 (MQ=255)                                                                         gTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                         >  2:254918/1‑97 (MQ=255)                                                                         gTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCt                                                                                             >  2:922100/1‑109 (MQ=255)                                                                         gTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCt                                                                                             <  1:922100/109‑1 (MQ=255)                                                                                 actgactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcgc                                                                                              >  1:198395/1‑100 (MQ=255)                                                                                 actgactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcgc                                                                                              <  2:198395/100‑1 (MQ=255)                                                                                    gactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              <  1:555232/81‑1 (MQ=255)                                                                                    gactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                              <  1:586233/113‑1 (MQ=255)                                                                                    gactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                              >  2:586233/1‑113 (MQ=255)                                                                                    gactgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAAAGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              >  2:555232/1‑81 (MQ=255)                                                                                     actgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTTTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATgg                                                   >  1:873843/1‑139 (MQ=255)                                                                                     actgTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATgg                                                   >  2:670141/1‑139 (MQ=255)                                                                                         tATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCa                                               >  2:1108150/1‑139 (MQ=255)                                                                                          aTTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                                              >  2:848158/1‑139 (MQ=255)                                                                                                   aaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAc                                      <  1:670141/139‑1 (MQ=255)                                                                                                    aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                     >  2:1088008/1‑139 (MQ=255)                                                                                                           aGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa                              <  1:1088008/139‑1 (MQ=255)                                                                                                            gTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTaa                             >  2:512193/1‑139 (MQ=255)                                                                                                             tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                                             <  2:113906/90‑1 (MQ=255)                                                                                                             tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                                             >  1:113906/1‑90 (MQ=255)                                                                                                             tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATg                                <  2:211195/135‑1 (MQ=255)                                                                                                             tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATg                                >  1:211195/1‑135 (MQ=255)                                                                                                             tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac                            >  1:337518/1‑139 (MQ=255)                                                                                                              gCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                     <  2:88239/129‑1 (MQ=255)                                                                                                              gCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                     >  1:88239/1‑129 (MQ=255)                                                                                                               cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTAGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAacac                          >  1:22512/1‑139 (MQ=255)                                                                                                                tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                         >  1:297355/1‑139 (MQ=255)                                                                                                                tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                         <  1:848158/139‑1 (MQ=255)                                                                                                                       aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCGATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCa                  >  1:226170/1‑139 (MQ=255)                                                                                                                       aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttgtt                                                                                    >  2:658112/1‑73 (MQ=255)                                                                                                                       aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttgtt                                                                                    <  1:658112/73‑1 (MQ=255)                                                                                                                       aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCa                                                              >  1:651919/1‑95 (MQ=255)                                                                                                                       aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCa                                                              <  2:651919/95‑1 (MQ=255)                                                                                                                         aaTGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              >  1:630415/1‑45 (MQ=255)                                                                                                                         aaTGTACCTGAAGATAGCCTTCATTTATACTCCAATGTTTCTTTa                                                                                                              <  2:630415/45‑1 (MQ=255)                                                                                                                                   aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAttttt      >  2:360122/1‑139 (MQ=255)                                                                                                                                       tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                         >  1:1066910/1‑116 (MQ=255)                                                                                                                                       tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCa  <  1:1108150/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCC‑ACAATAAAACTTGTATATGCACTGACTGTATTACAA‑CAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCA  >  NC_000913/150812‑151082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑