Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,120	G→A	*205* (TAG→TAA)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,120	0	G	A	100.0%	149.5 / NA	46	*205* (TAG→TAA)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCG  >  NC_000913/1664985‑1665255                                                                                                                                        |                                                                                                                                        acGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                      <  1:205168/139‑1 (MQ=255)           gATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata                                                                                                                            >  2:293263/1‑139 (MQ=255)                                 aaCACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                      >  2:479962/1‑139 (MQ=255)                                 aaCACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                      <  1:479962/139‑1 (MQ=255)                                              ccGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                         <  2:343130/139‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTTCCg                                                                                                                      >  1:63335/1‑108 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCg                                                                                                                      <  2:63335/108‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                       >  1:536389/1‑139 (MQ=255)                                                gTGAACTGGCTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTCAATTCCTGTCGCGGTTAAACCGCTGTGTCGATAAGATAACAGTAATATTGCCGCATGCTGACGATTACGCGCAATCCAGGaata                                                                                       >  2:220387/1‑139 (MQ=255)                                                   cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGAt                                                                                                          <  2:615451/117‑1 (MQ=255)                                                   cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGAt                                                                                                          >  1:615451/1‑117 (MQ=255)                                                    aCTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                       >  1:1001341/1‑135 (MQ=255)                                                    aCTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                       <  2:1001341/135‑1 (MQ=255)                                                       gggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCCATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGtt                                                                                <  2:996477/139‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCGATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                >  2:190720/1‑122 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTCcgcg                                                                                                    >  1:732003/1‑118 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcgcg                                                                                                    <  2:732003/118‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                <  1:190720/122‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg                                                                                             >  1:948592/1‑125 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg                                                                                             <  2:948592/125‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaataat                                                                                     <  2:953673/133‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                               >  2:885531/1‑139 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                               >  2:304075/1‑139 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCAGGATGGTGACGATTACGCGCAATCCGGCaataat                                                                                     >  1:953673/1‑133 (MQ=255)                                                           gAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAg                                                                            >  2:340806/1‑139 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                <  1:785448/117‑1 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                >  2:785448/1‑117 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                            >  1:763441/1‑121 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                            <  2:763441/121‑1 (MQ=255)                                                                   tCGCTTAACGCTGGCGCAATGGCAGTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata                                                                                                                            <  1:12046/83‑1 (MQ=255)                                                                   tCGCTTAACGCTGGCGCAATGGCAGTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata                                                                                                                            >  2:12046/1‑83 (MQ=255)                                                                      cTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttt                                                                 >  2:504797/1‑139 (MQ=255)                                                                                           aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCa                                                                                                                                    >  2:70701/1‑51 (MQ=255)                                                                                           aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCa                                                                                                                                    <  1:70701/51‑1 (MQ=255)                                                                                            tCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCg                                                                                                                                 <  2:576433/53‑1 (MQ=255)                                                                                            tCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCg                                                                                                                                 >  1:576433/1‑53 (MQ=255)                                                                                                         ttCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                              <  1:340806/139‑1 (MQ=255)                                                                                                             tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                              >  2:600354/1‑103 (MQ=255)                                                                                                             tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                              <  1:600354/103‑1 (MQ=255)                                                                                                              gTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                >  2:71152/1‑68 (MQ=255)                                                                                                              gTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                                <  1:71152/68‑1 (MQ=255)                                                                                                                    gTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                       >  2:539249/1‑71 (MQ=255)                                                                                                                    gTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                       <  1:539249/71‑1 (MQ=255)                                                                                                                               gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGTTTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAATCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt        >  1:189112/1‑139 (MQ=255)                                                                                                                                  tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc     <  2:565036/139‑1 (MQ=255)                                                                                                                                     aTAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCg  >  2:186876/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCG  >  NC_000913/1664985‑1665255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑