Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,018	C→T	*123* (TAG→TAA)	sufA ←	Fe‑S cluster assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,018	0	C	T	100.0%	56.1 / NA	19	*123* (TAG→TAA)	sufA	Fe‑S cluster assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/6);  total (13/6)

TTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGC  >  NC_000913/1763904‑1764147                                                                                                                   |                                                                                                                                  ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                          <  1:985766/139‑1 (MQ=255) ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                          >  2:865115/1‑139 (MQ=255)             cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                              >  1:1038885/1‑139 (MQ=255)                     aaCTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                                                      <  1:865115/139‑1 (MQ=255)                             gaagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                          >  2:1030286/1‑95 (MQ=255)                             gaagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                          <  1:1030286/95‑1 (MQ=255)                                  aTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattcatt                                                                                                <  1:154990/116‑1 (MQ=255)                                  aTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattcatt                                                                                                >  2:154990/1‑116 (MQ=255)                                                  cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg                                                         >  2:1146/1‑139 (MQ=255)                                                   aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATTCCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGt                                                        >  2:579580/1‑139 (MQ=255)                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                                                  <  1:636636/127‑1 (MQ=255)                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                                                  >  2:636636/1‑127 (MQ=255)                                                               gTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGGCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacgaacg                                            <  2:1038885/139‑1 (MQ=255)                                                                          gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc                                 >  1:914451/1‑139 (MQ=255)                                                                          gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc                                 >  1:467966/1‑139 (MQ=255)                                                                                           cAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAAc                 >  1:1114487/1‑139 (MQ=255)                                                                                            aGTATTACGAGACATAGTACCGCTTGTACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACg                >  2:308040/1‑139 (MQ=255)                                                                                                      gaCATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTg      >  2:1020653/1‑139 (MQ=255)                                                                                                          tAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGc  >  1:340294/1‑139 (MQ=255)                                                                                                                   |                                                                                                                                  TTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGC  >  NC_000913/1763904‑1764147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑