Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,004	T→C	R78R (CGT→CGC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,004	0	T	C	100.0%	125.8 / NA	36	R78R (CGT→CGC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

GTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862875‑1863137                                                                                                                                  |                                                                                                                                      gTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGACt                                                                                                                              >  2:901107/1‑139 (MQ=255)    aGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           <  2:368091/139‑1 (MQ=255)     gACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAcc                                                                                                                          <  2:1121594/139‑1 (MQ=255)                              tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGa                                                                                                 >  1:1082905/1‑139 (MQ=255)                              tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGa                                                                                                 >  2:426469/1‑139 (MQ=255)                              tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGATCATGTGATCGTTAACGGTAAAAAAATCCATGTTACCGCTGAACGCGATCCGGCTAACCTCAAATGGGACGAAGTTGGTGTTGa                                                                                                 >  2:747875/1‑139 (MQ=255)                               gAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGAc                                                                                                >  2:561467/1‑139 (MQ=255)                                 aaTATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGt                                                                                              <  2:1082905/139‑1 (MQ=255)                                                   gCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTT‑CCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  1:285515/1‑139 (MQ=255)                                                    ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:561467/139‑1 (MQ=255)                                                        ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCt                                                                                                                              <  2:342700/84‑1 (MQ=255)                                                        ttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCt                                                                                                                              >  1:342700/1‑84 (MQ=255)                                                                tACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaa                                                                                                                      >  1:1015546/1‑84 (MQ=255)                                                                tACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaa                                                                                                                      <  2:1015546/84‑1 (MQ=255)                                                                                  cGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGACTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             <  1:901107/139‑1 (MQ=255)                                                                                    gTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     >  1:228096/1‑129 (MQ=255)                                                                                    gTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  2:228096/129‑1 (MQ=255)                                                                                    gTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                >  1:482974/1‑134 (MQ=255)                                                                                    gTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGt                                                <  2:482974/134‑1 (MQ=255)                                                                                    gTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAaca                                           >  2:786907/1‑139 (MQ=255)                                                                                                 tAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGt                              >  2:217360/1‑139 (MQ=255)                                                                                                   aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGc                            <  2:833701/139‑1 (MQ=255)                                                                                                    cGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCg                           <  1:786907/139‑1 (MQ=255)                                                                                                      gTAATAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACGCATCACCGCTGGTGCgaa                         <  1:747875/139‑1 (MQ=255)                                                                                                      gTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaa                         <  1:426469/139‑1 (MQ=255)                                                                                                        aaaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  1:247399/109‑1 (MQ=255)                                                                                                        aaaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     >  2:247399/1‑109 (MQ=255)                                                                                                         aaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaagaa                      >  2:54067/1‑139 (MQ=255)                                                                                                          aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  2:632173/139‑1 (MQ=255)                                                                                                          aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     >  1:70078/1‑139 (MQ=255)                                                                                                           aaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAg                    <  2:70078/139‑1 (MQ=255)                                                                                                                 cGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTa              >  1:1032254/1‑139 (MQ=255)                                                                                                                          gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:54067/139‑1 (MQ=255)                                                                                                                           cTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     >  2:281265/1‑90 (MQ=255)                                                                                                                           cTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  1:281265/90‑1 (MQ=255)                                                                                                                             gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTTTCGCTGAAGCAACTGGTCTGTTCATGACTGACGAAACTGCTCCAAAACACATCACCGCTGGTGAGAAGAAAGTAGCTATGACTGGTCCGt  >  2:239168/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      GTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862875‑1863137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑