Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,325	A→G	T104T (ACT→ACC)	yeaE ←	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,325	0	A	G	100.0%	117.7 / NA	33	T104T (ACT→ACC)	yeaE	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

AGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCG  >  NC_000913/1865196‑1865457                                                                                                                                  |                                                                                                                                     aGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgaga                                                                                                                             <  1:324940/139‑1 (MQ=255)             gTTAGAAATGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTg                                                                                                                 >  2:486368/1‑139 (MQ=255)              ttAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              <  2:736570/125‑1 (MQ=255)              ttAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              >  1:736570/1‑125 (MQ=255)                 gAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAGGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              >  2:95774/1‑122 (MQ=255)                 gAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              <  1:95774/122‑1 (MQ=255)                      gCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCgcat                                                                                                        >  1:497007/1‑139 (MQ=255)                           aGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCAtt                                                                                                   >  1:176774/1‑139 (MQ=255)                                               gCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAg                                                                               <  2:732683/139‑1 (MQ=255)                                                          ttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                    <  1:486368/139‑1 (MQ=255)                                                           tCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                   <  2:114549/139‑1 (MQ=255)                                                            ccATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:497007/139‑1 (MQ=255)                                                            ccATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:176774/139‑1 (MQ=255)                                                                     cGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTaga                                                         >  1:943211/1‑139 (MQ=255)                                                                      gACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                        <  2:394326/139‑1 (MQ=255)                                                                           tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                         <  1:525175/117‑1 (MQ=255)                                                                           tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                         >  2:525175/1‑117 (MQ=255)                                                                                                        tgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAgaga                                                       >  1:304155/1‑106 (MQ=255)                                                                                                        tgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAgaga                                                       <  2:304155/106‑1 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              >  1:37318/1‑105 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              <  2:37318/105‑1 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTCATGCTTCCCCAac            >  1:1088566/1‑139 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCtt                   <  2:411390/132‑1 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCtt                   >  1:411390/1‑132 (MQ=255)                                                                                                                  aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAac            >  2:651636/1‑139 (MQ=255)                                                                                                                    aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:333496/83‑1 (MQ=255)                                                                                                                    aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  >  1:333496/1‑83 (MQ=255)                                                                                                                    aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              >  2:677539/1‑103 (MQ=255)                                                                                                                    aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              <  1:677539/103‑1 (MQ=255)                                                                                                                           gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                >  1:765537/1‑110 (MQ=255)                                                                                                                           gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                <  2:765537/110‑1 (MQ=255)                                                                                                                           gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTc   >  2:1073818/1‑139 (MQ=255)                                                                                                                            aTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCg  <  1:651636/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     AGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCG  >  NC_000913/1865196‑1865457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑