Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,782	G→A	*221* (TAG→TAA)  D686D (GAC→GAT)	exoX → ptrB ←	exodeoxyribonuclease 10, DNA exonuclease X protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,782	0	G	A	100.0%	134.6 / NA	42	*221* (TAG→TAA)  D686D (GAC→GAT)	exoX ptrB	exodeoxyribonuclease 10, DNA exonuclease X protease II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/20);  total (22/20)

TGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAG  >  NC_000913/1926647‑1926908                                                                                                                                        |                                                                                                                               tGGCAAATACCGTGGCAAAGCGGTTTCCGGCGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATcc                                                                                                                             >  1:99483/1‑139 (MQ=255)          ccGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTa                                                                                                                    >  2:1013437/1‑139 (MQ=255)             tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCg                                                                                                                 >  1:1037388/1‑139 (MQ=255)             tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCg                                                                                                                 >  2:628424/1‑139 (MQ=255)                                            gATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGaa                                                                                  <  2:1037388/139‑1 (MQ=255)                                                       tCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                       <  2:99483/139‑1 (MQ=255)                                                                     ttAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTc                                                         >  2:828105/1‑139 (MQ=255)                                                                        ataaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAc                                                                                       <  2:160485/106‑1 (MQ=255)                                                                        ataaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAc                                                                                       >  1:160485/1‑106 (MQ=255)                                                                               ggACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATc                                                                                                                              >  2:290912/1‑60 (MQ=255)                                                                               ggACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATc                                                                                                                              <  1:290912/60‑1 (MQ=255)                                                                               ggACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTa                                               >  1:630747/1‑139 (MQ=255)                                                                                 aCAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAgc                                                                                            >  1:107520/1‑92 (MQ=255)                                                                                 aCAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAgc                                                                                            <  2:107520/92‑1 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGt                                                                                                                     >  2:973814/1‑63 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGt                                                                                                                     <  1:973814/63‑1 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                  >  1:553747/1‑66 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                  <  2:553747/66‑1 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                       >  1:530100/1‑125 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                       <  2:530100/125‑1 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGa                                         >  2:636834/1‑139 (MQ=255)                                                                                     cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGa                                         >  2:621913/1‑139 (MQ=255)                                                                                          gCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGa                                    >  1:229773/1‑139 (MQ=255)                                                                                              ggAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGAGTTAAAGCGACCAGATTTa                                <  2:630747/139‑1 (MQ=255)                                                                                               gAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGaa                                                                                  >  1:747033/1‑88 (MQ=255)                                                                                               gAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGaa                                                                                  <  2:747033/88‑1 (MQ=255)                                                                                                aGCTACGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTAcc                              >  2:809859/1‑139 (MQ=255)                                                                                                    gCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCa                          <  1:1013437/139‑1 (MQ=255)                                                                                                     cGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGc                            >  2:1022258/1‑136 (MQ=255)                                                                                                     cGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGc                            <  1:1022258/136‑1 (MQ=255)                                                                                                     cGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCAt                         >  1:128506/1‑139 (MQ=255)                                                                                                               tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                  <  2:471032/40‑1 (MQ=39)                                                                                                               tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                  >  1:471032/1‑40 (MQ=255)                                                                                                               tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc               <  2:128506/139‑1 (MQ=255)                                                                                                               tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc               <  2:1086083/139‑1 (MQ=255)                                                                                                               tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc               >  1:947374/1‑139 (MQ=255)                                                                                                                    cATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt          <  1:828105/139‑1 (MQ=255)                                                                                                                    cATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt          <  1:621913/139‑1 (MQ=255)                                                                                                                            ggAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag  <  2:229773/139‑1 (MQ=255)                                                                                                                            ggAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag  <  1:809859/139‑1 (MQ=255)                                                                                                                                   aCTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGcc                           <  2:198420/107‑1 (MQ=255)                                                                                                                                   aCTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGcc                           >  1:198420/1‑107 (MQ=255)                                                                                                                                        |                                                                                                                               TGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAG  >  NC_000913/1926647‑1926908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑