Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,021,501 G→A *138* (TAG→TAA fliN → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,021,5010GA100.0% 102.2 / NA 34*138* (TAG→TAAfliNflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

GGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTT  >  NC_000913/2021378‑2021635
                                                                                                                           |                                                                                                                                      
ggATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                                         <  1:751445/139‑1 (MQ=255)
           tCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAatgatg                                                                                                                                   >  2:142892/1‑118 (MQ=255)
           tCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAatgatg                                                                                                                                   <  1:142892/118‑1 (MQ=255)
                          tCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGtt                                                                                               >  2:960834/1‑139 (MQ=255)
                                     gAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGGCTGAACGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                                                                                    <  2:985442/139‑1 (MQ=255)
                                                cGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAgg                                                                         >  2:432558/1‑139 (MQ=255)
                                                   tGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCGCGCTACTGTGCAATCTTCCGCGCCg                                                                                                  <  2:760154/111‑1 (MQ=255)
                                                   tGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCGCGCTACTGTGCAATCTTCCGCGCCg                                                                                                  >  1:760154/1‑111 (MQ=255)
                                                   tGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGa                                                                      >  1:673594/1‑139 (MQ=255)
                                                                      cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCat                                                   >  2:1093598/1‑139 (MQ=255)
                                                                                   tCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                                         <  1:181551/56‑1 (MQ=255)
                                                                                   tCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                                         >  2:181551/1‑56 (MQ=255)
                                                                                   tCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATc                                      >  1:809072/1‑139 (MQ=255)
                                                                                     aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                                                                                    >  1:333261/1‑91 (MQ=255)
                                                                                     aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                                                                                    <  2:333261/91‑1 (MQ=255)
                                                                                       tACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGa                                                                      <  1:478471/103‑1 (MQ=255)
                                                                                       tACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGa                                                                      >  2:478471/1‑103 (MQ=255)
                                                                                        aCTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgc                                 <  2:543626/139‑1 (MQ=255)
                                                                                           ccGTCTGAGCGAATGCGCCGCCTGAGCCGTTAAtg                                                                                                                                      >  1:794072/1‑35 (MQ=255)
                                                                                           ccGTCTGAGCGAATGCGCCGCCTGAGCCGTTAAtg                                                                                                                                      <  2:794072/35‑1 (MQ=255)
                                                                                             gTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGctgctg                            >  2:21553/1‑139 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAAt                                                                                                                                >  1:941309/1‑38 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAAt                                                                                                                                <  2:941309/38‑1 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAgcggcg                                                                <  1:803945/102‑1 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACt                                                            >  2:183352/1‑106 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACt                                                            <  1:183352/106‑1 (MQ=255)
                                                                                              tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACACTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAgcggcg                                                                >  2:803945/1‑102 (MQ=255)
                                                                                                  aGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCAc                                                                                  <  2:225534/80‑1 (MQ=255)
                                                                                                  aGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCAc                                                                                  >  1:225534/1‑80 (MQ=255)
                                                                                                        tGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                                         <  1:354305/35‑1 (MQ=255)
                                                                                                        tGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                                         >  2:354305/1‑35 (MQ=255)
                                                                                                        tGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTg                                         >  1:972548/1‑115 (MQ=255)
                                                                                                        tGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTg                                         <  2:972548/115‑1 (MQ=255)
                                                                                                               ccTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGt          <  1:960834/139‑1 (MQ=255)
                                                                                                                      cGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAtt   <  1:432558/139‑1 (MQ=255)
                                                                                                                       gTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAttt  >  2:1072980/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                      
GGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTT  >  NC_000913/2021378‑2021635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: