Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,792	G→A	T11M (ACG→ATG)	dsrB ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,792	0	G	A	100.0%	172.9 / NA	54	T11M (ACG→ATG)	dsrB	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

TACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCT  >  NC_000913/2024658‑2024915                                                                                                                                       |                                                                                                                            tACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCAtttt                                                                                                                         >  2:1018493/1‑139 (MQ=255)    cGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGAc                                                                                                                      <  1:987658/139‑1 (MQ=255)         tGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTa                                                                                                                 >  2:806339/1‑139 (MQ=255)          gATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTAc                                                                                                                >  2:462811/1‑139 (MQ=255)            tGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTAccc                                                                                                              <  1:806339/139‑1 (MQ=255)                cTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATc                                                                                                          >  2:158330/1‑139 (MQ=255)                             gAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTACTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATc                                                                                             >  1:674010/1‑139 (MQ=255)                                ccAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGtt                                                                                          >  1:1026169/1‑139 (MQ=255)                                 cAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTAcc                                                                                                               <  1:327462/117‑1 (MQ=255)                                 cAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTAcc                                                                                                               >  2:327462/1‑117 (MQ=255)                                  aGATGCCGAGCGGGTAGTCTTCCAGCTAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGtttt                                                                                        <  2:695469/139‑1 (MQ=255)                                   gATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTtc                                                                                       >  2:86600/1‑139 (MQ=255)                                         gAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCtg                                                                                 >  1:849523/1‑139 (MQ=255)                                          aGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCtgt                                                                                >  1:283814/1‑139 (MQ=255)                                              ggTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCt                                                                            <  1:462811/139‑1 (MQ=255)                                               gTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCtt                                                                           <  1:1018493/139‑1 (MQ=255)                                                    cTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                      <  1:60327/139‑1 (MQ=255)                                                    cTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                      <  2:283814/139‑1 (MQ=255)                                                      tCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCa                                                                    <  1:158330/139‑1 (MQ=255)                                                      tCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCAACTTCATCGTTTTCCTCCTGTGGCTTTGTGCCa                                                                    <  2:674010/139‑1 (MQ=255)                                                             aaaCCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGa                                                             >  1:1075906/1‑139 (MQ=255)                                                                     tACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTc                                                     <  1:86600/139‑1 (MQ=255)                                                                        aTTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGtt                                                  >  1:916148/1‑139 (MQ=255)                                                                              ccTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTg                                            >  1:763002/1‑139 (MQ=255)                                                                              ccTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTg                                            >  1:973047/1‑139 (MQ=255)                                                                                ttCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGc                                          <  2:1026169/139‑1 (MQ=255)                                                                                 tCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACTGCCATCCATTTTGAc                                                                                                                      <  2:758592/62‑1 (MQ=255)                                                                                 tCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGAc                                                                                                                      >  1:758592/1‑62 (MQ=255)                                                                                  cACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAg                                        >  2:672692/1‑139 (MQ=255)                                                                                      acaCTCCTCAACCGCCAGTACCACGCCAGGACGGCGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGATTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGcc                                    <  2:1075906/137‑1 (MQ=255)                                                                                            ctcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                      >  2:860413/1‑99 (MQ=255)                                                                                            ctcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                      <  1:860413/99‑1 (MQ=255)                                                                                            ctcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgc                              >  2:48737/1‑139 (MQ=255)                                                                                            ctcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgc                              >  1:368677/1‑139 (MQ=255)                                                                                             tcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgcg                             <  2:368677/139‑1 (MQ=255)                                                                                             tcAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATGTTGACt                                                                                                                     >  2:802463/1‑51 (MQ=255)                                                                                             tcAACGGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACt                                                                                                                     <  1:802463/51‑1 (MQ=255)                                                                                                 cTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGcgcgcgcg                         >  2:385910/1‑139 (MQ=255)                                                                                                 cTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGcgcgcgcg                         >  2:908695/1‑139 (MQ=255)                                                                                                       gTACCACGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCt                   >  2:831083/1‑139 (MQ=255)                                                                                                             cGCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGAtttt             >  2:473108/1‑139 (MQ=255)                                                                                                              gCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgcgcgc                          <  1:718084/125‑1 (MQ=255)                                                                                                              gCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgcgcgc                          >  2:718084/1‑125 (MQ=255)                                                                                                              gCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTtc            >  2:224163/1‑139 (MQ=255)                                                                                                              gCCAGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTtc            >  1:714854/1‑139 (MQ=255)                                                                                                                 aGGACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCg         <  1:48737/139‑1 (MQ=255)                                                                                                                  ggACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa        <  2:763002/139‑1 (MQ=255)                                                                                                                  ggACGACGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa        <  1:549091/139‑1 (MQ=255)                                                                                                                   gacgacGCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGAc       <  1:908695/139‑1 (MQ=255)                                                                                                                    acgacgCGGACCGTCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACg      <  2:916148/139‑1 (MQ=255)                                                                                                                     cgacgCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACgg     >  1:337022/1‑139 (MQ=255)                                                                                                                      gacgCGGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGt    <  2:849523/139‑1 (MQ=255)                                                                                                                       atgcgGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTc   <  1:245905/137‑1 (MQ=255)                                                                                                                        cgcgGACCGCCATCCATTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCt  >  1:401094/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                            TACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCT  >  NC_000913/2024658‑2024915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑