Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,215 C→T G251G (GGC→GGT)  cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,2150CT100.0% 117.8 / NA 37G251G (GGC→GGT) cdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

ATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTG  >  NC_000913/183087‑183339
                                                                                                                                |                                                                                                                            
aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                    >  1:651104/1‑139 (MQ=255)
                      tGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCg                                                                                              <  1:11477/139‑1 (MQ=255)
                                             gCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacga                                                                       <  2:651104/139‑1 (MQ=255)
                                                gaagaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTAcc                                                                                                                  >  1:114041/1‑93 (MQ=255)
                                                gaagaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTAcc                                                                                                                  <  2:114041/93‑1 (MQ=255)
                                                   gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGccc                                                                                               >  2:931022/1‑109 (MQ=255)
                                                   gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGccc                                                                                               <  1:931022/109‑1 (MQ=255)
                                                     tcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtg                                                               >  2:819824/1‑139 (MQ=255)
                                                       atcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggt                                                             <  1:819824/139‑1 (MQ=255)
                                                                   ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                 >  1:255763/1‑139 (MQ=255)
                                                                           cTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAg                                         >  1:512079/1‑139 (MQ=255)
                                                                            tGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGa                                        >  1:215901/1‑139 (MQ=255)
                                                                              aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                    <  2:143867/61‑1 (MQ=255)
                                                                              aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                    >  1:143867/1‑61 (MQ=255)
                                                                                tACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGt                                    >  1:209592/1‑139 (MQ=255)
                                                                                   ccGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgc                                 >  1:971130/1‑139 (MQ=255)
                                                                                     gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtg                                                                  <  1:682242/104‑1 (MQ=255)
                                                                                     gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtg                                                                  >  2:682242/1‑104 (MQ=255)
                                                                                     gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                               <  2:971130/139‑1 (MQ=255)
                                                                                     gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                               >  2:1132548/1‑139 (MQ=255)
                                                                                      cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCg                                                                                                                 >  2:1133586/1‑56 (MQ=255)
                                                                                      cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCg                                                                                                                 <  1:1133586/56‑1 (MQ=255)
                                                                                       aTGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCg                                                                                              >  2:1029054/1‑74 (MQ=255)
                                                                                       aTGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCg                                                                                              <  1:1029054/74‑1 (MQ=255)
                                                                                        tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                 <  1:801187/118‑1 (MQ=255)
                                                                                        tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                 >  2:801187/1‑118 (MQ=255)
                                                                                        tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                               <  1:126865/120‑1 (MQ=255)
                                                                                        tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                               >  2:126865/1‑120 (MQ=255)
                                                                                                tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                    <  2:512079/139‑1 (MQ=255)
                                                                                                     ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               <  2:160828/43‑1 (MQ=255)
                                                                                                     ccAGCTGCGTTTTCGAGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               >  1:160828/1‑43 (MQ=38)
                                                                                                       aGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCt             <  2:215901/139‑1 (MQ=255)
                                                                                                        gCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                                     <  1:134099/82‑1 (MQ=255)
                                                                                                        gCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                                     >  2:134099/1‑82 (MQ=255)
                                                                                                         cTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGa           <  1:1132548/139‑1 (MQ=255)
                                                                                                          tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt          >  1:337798/1‑139 (MQ=255)
                                                                                                                  cgcgTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCtg  <  2:209592/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                            
ATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTG  >  NC_000913/183087‑183339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑