Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,179,330	T→C	S170S (AGT→AGC)	yegT →	nucleoside transporter, low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,179,330	0	T	C	100.0%	113.8 / NA	32	S170S (AGT→AGC)	yegT	nucleoside transporter, low affinity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

CCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTT  >  NC_000913/2179201‑2179461                                                                                                                                  |                                                                                                                                    cccGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGctctgctctg                                                                                                                            <  2:745710/139‑1 (MQ=255)   cgcgCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTctgctctgct                                                                                                                          <  1:254691/139‑1 (MQ=255)       cATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGgt                                                                                                                      >  1:587796/1‑139 (MQ=255)                gATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCg                                                                                                             <  2:848592/139‑1 (MQ=255)                    ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         >  2:1093135/1‑139 (MQ=255)                                  gATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACg                                                                                           >  1:994419/1‑139 (MQ=255)                                          ccGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCaaa                                                                                   <  2:587796/139‑1 (MQ=255)                                            ggTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCaaaaa                                                                                 >  2:1001630/1‑139 (MQ=255)                                                  gCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCg                                                                           >  2:46204/1‑139 (MQ=255)                                                         gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                    <  1:1093135/139‑1 (MQ=255)                                                               tGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGatat                                                              <  1:979748/139‑1 (MQ=255)                                                                 ccGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTa                                                            >  2:932781/1‑139 (MQ=255)                                                                    cAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAg                                                         >  1:78187/1‑139 (MQ=255)                                                                            ggggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTc                                                 >  2:399796/1‑139 (MQ=255)                                                                                tATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                             <  1:932781/139‑1 (MQ=255)                                                                                 aTGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCtgctctgctc                                                                                                                         >  1:706293/1‑62 (MQ=255)                                                                                 aTGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCtgctctgctc                                                                                                                         <  2:706293/62‑1 (MQ=255)                                                                                  tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGtt                                                                                                                 <  2:1074566/69‑1 (MQ=255)                                                                                  tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGtt                                                                                                                 >  1:1074566/1‑69 (MQ=255)                                                                                  tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTg                                           >  1:727583/1‑139 (MQ=255)                                                                                            tcACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGa                                 >  2:895748/1‑139 (MQ=255)                                                                                              aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcca                                                                                        >  2:1078605/1‑82 (MQ=255)                                                                                              aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcca                                                                                        <  1:1078605/82‑1 (MQ=255)                                                                                               ccGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                               <  1:903755/90‑1 (MQ=255)                                                                                               ccGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                               >  2:903755/1‑90 (MQ=255)                                                                                                            cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACg                                                                                           <  2:393539/65‑1 (MQ=255)                                                                                                            cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACg                                                                                           >  1:393539/1‑65 (MQ=255)                                                                                                                    aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc         <  1:1001630/139‑1 (MQ=255)                                                                                                                    aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc         <  1:46204/139‑1 (MQ=255)                                                                                                                           ccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCtt  >  2:19022/1‑139 (MQ=255)                                                                                                                           ccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCtt  >  2:1072216/1‑139 (MQ=255)                                                                                                                              gAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                  <  1:1048856/88‑1 (MQ=255)                                                                                                                              gAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                  >  2:1048856/1‑88 (MQ=255)                                                                                                                                  |                                                                                                                                    CCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTT  >  NC_000913/2179201‑2179461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑