| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,268,531 | G→A | G235R (GGG→AGG) | yeiR → | Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,268,531 | 0 | G | A | 100.0% | 112.5 / NA | 35 | G235R (GGG→AGG) | yeiR | Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/19); total (16/19) | |||||||||||
TCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCT > NC_000913/2268395‑2268643 | tcttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:726740/1‑139 (MQ=255)tcttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:863228/1‑139 (MQ=255) tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 2:625214/1‑139 (MQ=255) tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 1:297165/1‑139 (MQ=255) tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 2:142287/1‑139 (MQ=255) gCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAg > 2:460470/1‑139 (MQ=255) gcgTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGcc < 1:286686/139‑1 (MQ=255) cGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCg < 1:917172/139‑1 (MQ=255) ccGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATAtt < 1:313711/139‑1 (MQ=255) ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc > 2:548222/1‑135 (MQ=255) ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc < 1:548222/135‑1 (MQ=255) ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACg < 1:625214/139‑1 (MQ=255) gCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc > 1:30661/1‑133 (MQ=255) gCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc < 2:30661/133‑1 (MQ=255) gCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa < 1:142287/139‑1 (MQ=255) cAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg < 1:1111641/139‑1 (MQ=255) catcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg < 2:726740/139‑1 (MQ=255) gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTgg > 1:1030621/1‑139 (MQ=255) aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGc > 2:946499/1‑100 (MQ=255) aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGc < 1:946499/100‑1 (MQ=255) gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg < 2:579408/49‑1 (MQ=255) gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:579408/1‑49 (MQ=255) gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTg > 1:885587/1‑139 (MQ=255) aGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAAc > 2:1000841/1‑139 (MQ=255) gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg > 1:678695/1‑82 (MQ=255) gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg < 2:678695/82‑1 (MQ=255) gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg < 1:739519/98‑1 (MQ=255) gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTc > 1:397543/1‑139 (MQ=255) gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGCATATTCGACGCTGATACGGTATTCGACACCATTg > 2:739519/1‑98 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAATGATATCAGGCCTGCGGCTGGATATTCGa < 1:703450/67‑1 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGa > 2:703450/1‑67 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa > 2:582989/1‑85 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa < 1:582989/85‑1 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa > 2:808251/1‑85 (MQ=255) agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa < 1:808251/85‑1 (MQ=255) cATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCg < 2:885587/139‑1 (MQ=255) tCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtg < 2:863228/139‑1 (MQ=255) cAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtgc < 2:397543/139‑1 (MQ=255) aaCGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtgct < 2:297165/139‑1 (MQ=255) | TCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCT > NC_000913/2268395‑2268643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |