Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,166	A→G	K601K (AAA→AAG)	yejA →	microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,166	0	A	G	100.0%	178.9 / NA	49	K601K (AAA→AAG)	yejA	microcin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/22);  total (27/22)

TGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGAC  >  NC_000913/2274032‑2274286                                                                                                                                       |                                                                                                                         tgGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                      <  2:232625/139‑1 (MQ=255)         ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaa                                                                                                              >  2:609808/1‑138 (MQ=255)                                           tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                       <  1:760638/128‑1 (MQ=255)                                           tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                       >  2:760638/1‑128 (MQ=255)                                           tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                            >  1:462531/1‑139 (MQ=255)                                                          cgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                             >  1:339869/1‑139 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaa                                                                                                             <  2:985767/74‑3 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaa                                                                                                             >  1:985767/1‑72 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                     >  2:104931/1‑98 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                     <  1:104931/98‑1 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           <  2:365850/108‑1 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           >  1:365850/1‑108 (MQ=255)                                                                           cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                            <  2:339869/139‑1 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcgc                                                                                                      >  2:868084/1‑80 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcgc                                                                                                      <  1:868084/80‑1 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                       <  1:1107909/79‑1 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                       >  2:1107909/1‑79 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           >  1:591442/1‑107 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           <  2:591442/107‑1 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                           >  1:774609/1‑139 (MQ=255)                                                                            ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                           >  2:928640/1‑139 (MQ=255)                                                                             gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                             >  1:614448/1‑88 (MQ=255)                                                                             gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                             <  2:614448/88‑1 (MQ=255)                                                                                   aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                       >  2:990527/1‑88 (MQ=255)                                                                                   aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                       <  1:990527/88‑1 (MQ=255)                                                                                        tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATg                                                                                                           <  1:300374/63‑1 (MQ=255)                                                                                        tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATg                                                                                                           >  2:300374/1‑63 (MQ=255)                                                                                          gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                      <  1:133563/50‑1 (MQ=255)                                                                                          gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                      >  2:133563/1‑50 (MQ=255)                                                                                          gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTg                             >  2:1127506/1‑139 (MQ=255)                                                                                           tggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                         >  2:417064/1‑94 (MQ=255)                                                                                           tggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                         <  1:417064/94‑1 (MQ=255)                                                                                             gtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                                                    >  1:802515/1‑113 (MQ=255)                                                                                             gtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCCGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                                                    <  2:802515/113‑1 (MQ=255)                                                                                                tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                    >  1:669628/1‑46 (MQ=255)                                                                                                tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                    <  2:669628/46‑1 (MQ=255)                                                                                                    gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTg                             <  2:610626/129‑1 (MQ=255)                                                                                                    gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTgcgc                   >  1:773836/1‑139 (MQ=255)                                                                                                    gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAATCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTg                             >  1:610626/1‑129 (MQ=255)                                                                                                     tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGcc                  >  1:306683/1‑139 (MQ=255)                                                                                                       aaTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTg                >  1:195766/1‑139 (MQ=255)                                                                                                              cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           >  2:616627/1‑73 (MQ=255)                                                                                                              cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                           <  1:616627/73‑1 (MQ=255)                                                                                                               ggCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccg        <  2:462531/139‑1 (MQ=255)                                                                                                                gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg       <  2:774609/139‑1 (MQ=255)                                                                                                                 ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccggt      >  1:317827/1‑139 (MQ=255)                                                                                                                     aCTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCACCATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGAc  <  2:773836/139‑1 (MQ=255)                                                                                                                                   gCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                              >  2:145779/1‑81 (MQ=255)                                                                                                                                   gCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                              <  1:145779/81‑1 (MQ=255)                                                                                                                                       |                                                                                                                         TGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGAC  >  NC_000913/2274032‑2274286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑