Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,333,691	A→G	pseudogene (712/4605 nt)	yfaS ←	pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,333,691	0	A	G	100.0%	158.7 / NA	45	pseudogene (712/4605 nt)	yfaS	pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/25);  total (20/25)

GGCTTCGCTTCACCCTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGA  >  NC_000913/2333555‑2333825                                                                                                                                         |                                                                                                                                       ggCTTCGCTTCACCCTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCa                                                                                                                                      >  2:632866/1‑139 (MQ=255)           cACCCTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGctc                                                                                                                            >  2:1064574/1‑139 (MQ=255)                    ttttACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGcc                                                                                                                   <  1:152752/139‑1 (MQ=255)                       tACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:806440/139‑1 (MQ=255)                         cccGCGGTCCACACCAGAAGCTCTATGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  1:632866/139‑1 (MQ=255)                            gcgGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  1:459264/1‑134 (MQ=255)                            gcgGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:459264/134‑1 (MQ=255)                            gcgGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGaa                                                                                                           >  2:951818/1‑139 (MQ=255)                            gcgGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGaa                                                                                                           >  2:697822/1‑139 (MQ=255)                                              tctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCATCCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCt                                                                                         <  2:254531/139‑1 (MQ=255)                                               ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTg                                                                                        >  2:461616/1‑139 (MQ=255)                                               ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTg                                                                                        >  2:1029047/1‑139 (MQ=255)                                                  tGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     <  1:697822/139‑1 (MQ=255)                                                  tGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     <  1:1029047/139‑1 (MQ=255)                                                     cTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCga                                                                                  >  1:133463/1‑139 (MQ=255)                                                            tttGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  1:560222/1‑102 (MQ=255)                                                            tttGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:560222/102‑1 (MQ=255)                                                              tGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              >  2:960285/1‑102 (MQ=255)                                                              tGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  1:960285/102‑1 (MQ=255)                                                               gCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                                        <  2:888408/139‑1 (MQ=255)                                                                        cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  2:576535/1‑90 (MQ=255)                                                                        cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:576535/90‑1 (MQ=255)                                                                        cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               >  1:370444/1‑139 (MQ=255)                                                                          cGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                                             >  1:1018975/1‑139 (MQ=255)                                                                             tATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCAGCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTc                                                          <  1:873393/139‑1 (MQ=255)                                                                              aTCGGAAACAAACACCACCGTCTTCGCCCGATACACACCAAACATCGCCTCGACGAGGGGCAGTCCCGGCTCTTGTTGGCCGGGAGGAATATAAAGGTTACGCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                                         <  2:626852/139‑1 (MQ=255)                                                                              aTCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                                         <  2:133463/139‑1 (MQ=255)                                                                               tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCa                                                        >  1:554527/1‑139 (MQ=255)                                                                                     acaaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTa                                                  >  1:849290/1‑139 (MQ=255)                                                                                       aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  1:456249/1‑75 (MQ=255)                                                                                       aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:456249/75‑1 (MQ=255)                                                                                                cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgctgc                                       <  1:513075/139‑1 (MQ=255)                                                                                                 gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCg                                      >  2:780467/1‑139 (MQ=255)                                                                                                    gtcgCCCGATAC‑‑‑CCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa                                <  1:71475/139‑1 (MQ=255)                                                                                                       gCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa                                <  2:370444/139‑1 (MQ=255)                                                                                                       gCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa                                <  2:554527/139‑1 (MQ=255)                                                                                                           gATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACgg                            >  2:1079612/1‑139 (MQ=255)                                                                                                               cccACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTa                        <  1:780467/139‑1 (MQ=255)                                                                                                                  accaaccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCgag                                                                                 <  2:237632/79‑1 (MQ=255)                                                                                                                  accaaccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCgag                                                                                 >  1:237632/1‑79 (MQ=255)                                                                                                                       ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCa                <  1:1064574/139‑1 (MQ=255)                                                                                                                          tCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTg                   <  1:645837/133‑1 (MQ=255)                                                                                                                          tCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTg                   >  2:645837/1‑133 (MQ=255)                                                                                                                                     aGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGa  <  1:951818/139‑1 (MQ=255)                                                                                                                                     aGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAAATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGa  <  2:1018975/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCTTCGCTTCACCCTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGA  >  NC_000913/2333555‑2333825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑