Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,348	C→T	*432* (TAG→TAA)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,348	0	C	T	100.0%	78.2 / NA	25	*432* (TAG→TAA)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379218‑2379482                                                                                                                                   |                                                                                                                                       aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:423806/139‑1 (MQ=255) aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:415953/139‑1 (MQ=255)      ccgccgCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGta                                                                                                                           <  2:839074/139‑1 (MQ=255)                      gTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                >  2:234568/1‑118 (MQ=255)                      gTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:234568/118‑1 (MQ=255)                                   aCTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGACGCTttgttg                                                                                              >  1:1086614/1‑139 (MQ=255)                                                 gTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCa                                                                                >  1:345551/1‑139 (MQ=255)                                                  tCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTg                                                                                                         >  1:170631/1‑113 (MQ=255)                                                  tCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTg                                                                                                         <  2:170631/113‑1 (MQ=255)                                                       tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTg                                                                          >  1:426541/1‑139 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  1:368134/1‑139 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                <  2:975019/139‑1 (MQ=255)                                                                                  gCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGAttt                                                                                       >  1:235682/1‑99 (MQ=255)                                                                                  gCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGAttt                                                                                       <  2:235682/99‑1 (MQ=255)                                                                                    tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGTTCGGGGTCGGAACCACGGACAATGCCcg                                             >  2:1120248/1‑139 (MQ=255)                                                                                    tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  1:394575/1‑139 (MQ=255)                                                                                                      atatGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCa                           <  2:426541/139‑1 (MQ=255)                                                                                                           aaTCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       <  1:12362/90‑1 (MQ=255)                                                                                                           aaTCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       >  2:12362/1‑90 (MQ=255)                                                                                                                 tAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCg                <  1:1120248/139‑1 (MQ=255)                                                                                                                       tgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                          >  2:837945/1‑91 (MQ=255)                                                                                                                       tgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                          <  1:837945/91‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                          <  1:875943/87‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                          >  2:875943/1‑87 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg      >  1:685554/1‑139 (MQ=255)                                                                                                                               attattattCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGa  >  2:939653/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379218‑2379482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑