Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,105	G→A	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS → yfdK ←	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,105	0	G	A	100.0%	206.4 / NA	63	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS yfdK	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/38);  total (25/38)

AATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTA  >  NC_000913/2470969‑2471236                                                                                                                                         |                                                                                                                                    aaTTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGc                                                                                                                                   <  2:101521/139‑1 (MQ=255) aaTTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGc                                                                                                                                   <  2:938538/139‑1 (MQ=255)    tACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCAt                                                                                                                                <  2:470731/139‑1 (MQ=255)               cGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTgg                                                                                                                     <  2:4203/139‑1 (MQ=255)                 gAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcg                                                                                                                   <  1:437819/139‑1 (MQ=255)                  aaGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCGTCTGGCCTGAACTGCAGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                                  <  2:655692/139‑1 (MQ=255)                             ggcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                       >  2:151744/1‑139 (MQ=255)                              gcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGTGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATAGCTGGCGCACTGGAAGTATc                                                                                                      >  2:844724/1‑139 (MQ=255)                              gcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATc                                                                                                      >  2:759222/1‑139 (MQ=255)                                 gagaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                   <  2:861363/139‑1 (MQ=255)                                 gagaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                   <  1:844724/139‑1 (MQ=255)                                 gagaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                   <  1:649314/139‑1 (MQ=255)                                  agaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAcc                                                                                                  <  1:179435/139‑1 (MQ=255)                                        cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGc                                                                                                                                   >  1:879465/1‑100 (MQ=255)                                         ccGACTTAACACTGTGCTGGATTACATTGACGCGGCGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGCAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCa                                                                                           <  2:800927/139‑1 (MQ=255)                                          cGACTTAACACTGTGCTGGATTACATTGACGCGGTGATGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                          <  2:724246/139‑1 (MQ=255)                                          cGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                          <  1:282393/139‑1 (MQ=255)                                            aCTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTg                                                                                        >  1:948095/1‑139 (MQ=255)                                              ttAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCg                                                                                      <  1:337902/139‑1 (MQ=255)                                                   acTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAg                                                                                 <  2:654924/139‑1 (MQ=255)                                                       tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                             >  1:124371/1‑139 (MQ=255)                                                              ttACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGTCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCTCTGGATGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                      <  1:759222/139‑1 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTgg                                                                                                             <  2:732356/97‑1 (MQ=21)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTgg                                                                                                             >  1:732356/1‑97 (MQ=18)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                   <  2:873457/107‑1 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                   >  1:873457/1‑107 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATc                                                                           >  2:482718/1‑131 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATc                                                                           <  1:482718/131‑1 (MQ=255)                                                                                      aGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                       >  2:1132299/1‑114 (MQ=255)                                                                                      aGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                       <  1:1132299/114‑1 (MQ=255)                                                                                               cACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCa                                     >  2:425519/1‑139 (MQ=255)                                                                                                aCCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                        <  1:354024/103‑1 (MQ=255)                                                                                                aCCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                        >  2:354024/1‑103 (MQ=255)                                                                                                  cgcgCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgac                                  >  1:219896/1‑139 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTgg                                                                                                                     <  2:793479/54‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTgg                                                                                                                     >  1:793479/1‑54 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGaa                                                                                                           <  1:988357/64‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGaa                                                                                                           >  2:988357/1‑64 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                         >  2:451239/1‑82 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                         <  1:451239/82‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                      <  2:501010/101‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                      >  1:501010/1‑101 (MQ=255)                                                                                                            gTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                       <  2:996672/60‑1 (MQ=255)                                                                                                            gTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                       >  1:996672/1‑60 (MQ=255)                                                                                                               aTCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGc                     <  1:284637/139‑1 (MQ=255)                                                                                                               aTCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGc                     <  1:425519/139‑1 (MQ=255)                                                                                                                   ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAAt                                                                            >  2:858012/1‑80 (MQ=255)                                                                                                                   ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAAt                                                                            <  1:858012/80‑1 (MQ=255)                                                                                                                    gCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTAc                <  1:151744/139‑1 (MQ=255)                                                                                                                    gCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTAc                >  2:105643/1‑139 (MQ=255)                                                                                                                        gAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGc            <  1:60150/139‑1 (MQ=255)                                                                                                                         aaCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                       <  1:225263/47‑1 (MQ=255)                                                                                                                         aaCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                       >  2:225263/1‑47 (MQ=255)                                                                                                                          aCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGccc          <  1:418817/139‑1 (MQ=255)                                                                                                                           cTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                          <  2:128097/58‑1 (MQ=255)                                                                                                                           cTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                          >  1:128097/1‑58 (MQ=255)                                                                                                                           cTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCa         <  1:802206/139‑1 (MQ=255)                                                                                                                              ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTg      >  1:414426/1‑139 (MQ=255)                                                                                                                              ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTg      >  1:193789/1‑139 (MQ=255)                                                                                                                                 gaggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGttt   <  2:124371/139‑1 (MQ=255)                                                                                                                                  aggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTa  <  2:414426/139‑1 (MQ=255)                                                                                                                                   ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                       >  1:1101052/1‑69 (MQ=255)                                                                                                                                   ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                       <  2:1101052/69‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTA  >  NC_000913/2470969‑2471236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑