Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,910	G→A	A26T (GCC→ACC)	hmp →	fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,910	0	G	A	100.0%	175.9 / NA	55	A26T (GCC→ACC)	hmp	fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

GATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAAT  >  NC_000913/2685788‑2686035                                                                                                                           |                                                                                                                              gATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                               >  2:699477/1‑139 (MQ=255)   tGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTa                                                                                                             <  2:316586/139‑1 (MQ=255)                cATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                          <  1:525343/129‑1 (MQ=255)                cATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                          >  2:525343/1‑129 (MQ=255)                 aTCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAc                                                                                               >  1:130142/1‑139 (MQ=255)                        aaGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                                     >  2:933793/1‑110 (MQ=255)                        aaGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                                     <  1:933793/110‑1 (MQ=255)                           aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTAt                                                                                                            >  1:889242/1‑116 (MQ=255)                           aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTAt                                                                                                            <  2:889242/116‑1 (MQ=255)                           aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                                     >  1:305532/1‑139 (MQ=255)                                       gAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                            <  1:559609/88‑1 (MQ=255)                                       gAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                            >  2:559609/1‑88 (MQ=255)                                        aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                        <  1:724313/139‑1 (MQ=255)                                                 tGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                         <  1:624592/97‑1 (MQ=255)                                                 tGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                         >  2:624592/1‑97 (MQ=255)                                                  gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATg                                                                    <  1:955705/133‑1 (MQ=255)                                                  gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATg                                                                    >  2:955705/1‑133 (MQ=255)                                                  gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                              <  1:621427/139‑1 (MQ=255)                                                     tGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                               <  2:654502/87‑1 (MQ=255)                                                     tGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                               >  1:654502/1‑87 (MQ=255)                                                      gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                               >  2:943894/1‑86 (MQ=255)                                                      gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                               <  1:943894/86‑1 (MQ=255)                                                      gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGc                                                          >  1:313724/1‑139 (MQ=255)                                                       aCGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTa                                                                                                             >  1:77517/1‑87 (MQ=255)                                                       aCGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTa                                                                                                             <  2:77517/87‑1 (MQ=255)                                                       aCGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCg                                                         >  1:527460/1‑139 (MQ=255)                                                       aCGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCg                                                         <  2:130142/139‑1 (MQ=255)                                                           tCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAAt                                                     >  2:812176/1‑139 (MQ=255)                                                            cAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                 >  1:789814/1‑78 (MQ=255)                                                            cAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                 <  2:789814/78‑1 (MQ=255)                                                                     gCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                        >  2:268036/1‑78 (MQ=255)                                                                     gCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                        <  1:268036/78‑1 (MQ=255)                                                                         cAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGa                                       >  2:681652/1‑139 (MQ=255)                                                                             aaaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                   <  1:681652/139‑1 (MQ=255)                                                                              aaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                              >  2:87042/1‑111 (MQ=255)                                                                              aaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                              <  1:87042/111‑1 (MQ=255)                                                                                   caccaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGttt                             <  2:313724/139‑1 (MQ=255)                                                                                    accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTa                            >  1:864983/1‑139 (MQ=255)                                                                                      caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTAc                                                                                                                    >  2:670182/1‑49 (MQ=255)                                                                                      caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTAc                                                                                                                    <  1:670182/49‑1 (MQ=255)                                                                                        tCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                              >  1:831868/1‑101 (MQ=255)                                                                                        tCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                              <  2:831868/101‑1 (MQ=255)                                                                                                  ggtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc              <  1:699477/139‑1 (MQ=255)                                                                                                   gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                         >  1:816285/1‑47 (MQ=255)                                                                                                   gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                         <  2:816285/47‑1 (MQ=255)                                                                                                    tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                        >  1:767503/1‑79 (MQ=255)                                                                                                    tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                        <  2:767503/79‑1 (MQ=255)                                                                                                    tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTa            >  2:450920/1‑139 (MQ=255)                                                                                                      gAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGa                                                                                 <  1:1068017/68‑1 (MQ=255)                                                                                                      gAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGa                                                                                 >  2:1068017/1‑68 (MQ=255)                                                                                                             ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTaa   <  1:812176/139‑1 (MQ=255)                                                                                                             ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGGAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTaa   >  2:810329/1‑139 (MQ=255)                                                                                                              gCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCa                                                            <  1:277136/81‑1 (MQ=255)                                                                                                              gCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCa                                                            >  2:277136/1‑81 (MQ=255)                                                                                                              gCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAat  >  2:834759/1‑139 (MQ=255)                                                                                                                           |                                                                                                                              GATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAAT  >  NC_000913/2685788‑2686035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑