| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,706,879 | T→C | V82V (GTA→GTG) | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,706,879 | 0 | T | C | 100.0% | 144.2 / NA | 41 | V82V (GTA→GTG) | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/20); total (21/20) | |||||||||||
TTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTGCG > NC_000913/2706750‑2707012 | ttCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACACCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc > 1:831310/1‑139 (MQ=255) agaTCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCgggg < 2:479122/139‑1 (MQ=255) cATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCg > 2:431845/1‑139 (MQ=255) ttCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc < 2:807778/127‑1 (MQ=255) ttCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc > 1:807778/1‑127 (MQ=255) tGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGAGTCGATAAAGtt > 2:380982/1‑139 (MQ=255) tgtAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTg > 2:1049496/1‑139 (MQ=255) tGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg > 1:805692/1‑139 (MQ=255) tGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg > 1:339055/1‑139 (MQ=255) ccAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGcc > 1:548182/1‑139 (MQ=255) gTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAg < 1:431845/139‑1 (MQ=255) cTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCAc < 2:1121080/82‑1 (MQ=255) tACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCtt < 2:534983/134‑1 (MQ=255) tACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCtt > 1:534983/1‑134 (MQ=255) cGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGt < 1:1111151/123‑1 (MQ=255) cGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGt > 2:1111151/1‑123 (MQ=255) ctcGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTc > 2:690854/3‑139 (MQ=255) cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg < 1:278812/112‑1 (MQ=255) cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg > 2:278812/1‑112 (MQ=255) cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTc > 1:531611/1‑139 (MQ=255) cGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgct < 1:128083/139‑1 (MQ=255) cGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg < 1:785503/139‑1 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCt < 1:260337/114‑1 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCt > 2:260337/1‑114 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg > 1:865837/1‑139 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg < 2:865837/139‑1 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg < 2:491724/139‑1 (MQ=255) accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg > 1:491724/1‑139 (MQ=255) caGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCTCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg < 2:586152/132‑1 (MQ=255) caGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCTCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg > 1:586152/1‑132 (MQ=255) tCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCcac > 1:139333/1‑139 (MQ=255) caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc > 2:930527/1‑48 (MQ=255) caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc < 1:930527/48‑1 (MQ=255) caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCcacgct < 1:1049496/139‑1 (MQ=255) ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGa < 2:24144/51‑1 (MQ=255) ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGa > 1:24144/1‑51 (MQ=255) ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCacgctcacgctc < 2:831310/139‑1 (MQ=255) cagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCAcgctcacgctca < 2:339055/139‑1 (MQ=255) cagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCAcgctcacgctca < 1:951169/139‑1 (MQ=255) cGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTa < 2:222146/118‑1 (MQ=255) cGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTa > 1:222146/1‑118 (MQ=255) aaGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTgcg > 2:290841/1‑139 (MQ=255) | TTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTGCG > NC_000913/2706750‑2707012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |