Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,065	G→A	A113A (GCG→GCA)	pssA →	phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,065	0	G	A	100.0%	132.6 / NA	42	A113A (GCG→GCA)	pssA	phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/20);  total (22/20)

ACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT  >  NC_000913/2722932‑2723199                                                                                                                                      |                                                                                                                                       aCGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGa                                                                                                                                   >  1:1128266/1‑139 (MQ=255)      ttGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              <  2:968301/139‑1 (MQ=255)       tGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              <  2:416145/138‑1 (MQ=255)       tGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              >  1:416145/1‑138 (MQ=255)                   aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt                                                                                                                 >  1:160056/1‑139 (MQ=255)                              ccGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                      <  2:969206/139‑1 (MQ=255)                              ccGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                      <  2:346599/139‑1 (MQ=255)                                       gATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaa                                                                                             >  2:533310/1‑139 (MQ=255)                                       gATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaa                                                                                             >  1:5402/1‑139 (MQ=255)                                           tgCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGt                                                                                                           <  2:431681/121‑1 (MQ=255)                                           tgCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGt                                                                                                           >  1:431681/1‑121 (MQ=255)                                            gCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaat                                                                                        >  1:285179/1‑139 (MQ=255)                                                 tGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 >  2:127119/1‑125 (MQ=255)                                                 tGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 <  1:127119/125‑1 (MQ=255)                                                   cTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTg                                                                                 <  2:160056/139‑1 (MQ=255)                                                       tCGACTGGCATCGTGCACAACGTTGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                             <  1:373813/139‑1 (MQ=255)                                                         gACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGccc                                                                           <  1:533310/139‑1 (MQ=255)                                                                    tGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTg                                                                >  2:990239/1‑139 (MQ=255)                                                                     gCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGgtg                                                                     >  1:275358/1‑133 (MQ=255)                                                                     gCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc                                                               <  1:990239/139‑1 (MQ=255)                                                                     gCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGAATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGgtg                                                                     <  2:275358/133‑1 (MQ=255)                                                                           cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCg                                                                                                                            >  1:764288/1‑72 (MQ=255)                                                                           cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCg                                                                                                                            <  2:764288/72‑1 (MQ=255)                                                                           cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTa                                                         >  1:969167/1‑139 (MQ=255)                                                                                    aTTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTa                                                <  2:969167/139‑1 (MQ=255)                                                                                                     tAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTa                               <  2:5402/139‑1 (MQ=255)                                                                                                             aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat                                                                                            >  1:880689/1‑70 (MQ=255)                                                                                                             aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat                                                                                            <  2:880689/70‑1 (MQ=255)                                                                                                             aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                             <  2:5939/85‑1 (MQ=255)                                                                                                             aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                             >  1:5939/1‑85 (MQ=255)                                                                                                             aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                       >  2:491341/1‑139 (MQ=255)                                                                                                                 tGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg                                                                        <  2:711487/86‑1 (MQ=255)                                                                                                                 tGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg                                                                        >  1:711487/1‑86 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               <  1:35803/35‑1 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               >  2:35803/1‑35 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               >  2:782590/1‑35 (MQ=38)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               <  1:782590/35‑1 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg                                 <  2:197289/113‑1 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg                                 >  1:197289/1‑113 (MQ=255)                                                                                                                             ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAc       >  1:1073694/1‑139 (MQ=255)                                                                                                                               gCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGa     >  2:419062/1‑139 (MQ=255)                                                                                                                                  tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGt  >  1:298452/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       ACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT  >  NC_000913/2722932‑2723199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑