Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,421	T→C	P197P (CCT→CCC)	nadK →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,421	0	T	C	100.0%	129.1 / NA	36	P197P (CCT→CCC)	nadK	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

GTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAT  >  NC_000913/2751296‑2751555                                                                                                                              |                                                                                                                                       gTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGa                                                                                                                                 >  2:409756/1‑133 (MQ=255) gTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGa                                                                                                                                 <  1:409756/133‑1 (MQ=255)     cgcATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCctctct                                                                                                                       >  1:448951/1‑139 (MQ=255)                            tataTCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCa                                                                                                >  1:333749/1‑139 (MQ=255)                               atCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGt                                                                                             <  2:259950/139‑1 (MQ=255)                                  gacgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTcc                                                                                          <  1:747579/139‑1 (MQ=255)                                    cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      >  2:264864/1‑125 (MQ=255)                                    cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      <  1:264864/125‑1 (MQ=255)                                    cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              >  1:1089432/1‑133 (MQ=255)                                    cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              <  2:1089432/133‑1 (MQ=255)                                    cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCg                                                                                        >  2:711100/1‑139 (MQ=255)                                                                gATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              <  2:875391/105‑1 (MQ=255)                                                                gATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              >  1:875391/1‑105 (MQ=255)                                                                    gACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGt                                                                                                     >  1:506656/1‑94 (MQ=255)                                                                    gACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGt                                                                                                     <  2:506656/94‑1 (MQ=255)                                                                      cTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATAc                                                                                   <  1:30775/110‑1 (MQ=255)                                                                      cTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATAc                                                                                   >  2:30775/1‑110 (MQ=255)                                                                         attattTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagca                                                   <  1:490268/139‑1 (MQ=255)                                                                               tCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAgcagcagca                                             >  2:477622/1‑139 (MQ=255)                                                                                   cGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGAt                                         >  2:341291/1‑139 (MQ=255)                                                                                                  ccGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGAc                                                                                                                                <  1:485339/37‑1 (MQ=255)                                                                                                  ccGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGAc                                                                                                                                >  2:485339/1‑37 (MQ=255)                                                                                                      cTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                      >  1:691728/1‑139 (MQ=255)                                                                                                       tATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                     >  1:914538/1‑139 (MQ=255)                                                                                                             ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTTGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  2:448951/139‑1 (MQ=255)                                                                                                             ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  1:477622/139‑1 (MQ=255)                                                                                                             ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  1:341291/139‑1 (MQ=255)                                                                                                              tctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              <  2:914538/139‑1 (MQ=255)                                                                                                                 ctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa           >  2:581424/1‑139 (MQ=255)                                                                                                                 ctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa           >  2:90759/1‑139 (MQ=255)                                                                                                                   gCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAcc         <  1:581424/139‑1 (MQ=255)                                                                                                                    cAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      >  2:22378/1‑45 (MQ=255)                                                                                                                    cAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      <  1:22378/45‑1 (MQ=255)                                                                                                                          gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              >  2:260211/1‑127 (MQ=255)                                                                                                                          gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              <  1:260211/127‑1 (MQ=255)                                                                                                                          gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAt  >  1:766953/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                       GTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAT  >  NC_000913/2751296‑2751555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑