Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,413	A→G	K8E (AAA→GAA)	srlE →	glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,413	0	A	G	100.0%	118.0 / NA	33	K8E (AAA→GAA)	srlE	glucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

TCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGG  >  NC_000913/2826281‑2826539                                                                                                                                     |                                                                                                                               tctggtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGaa                                                                                                                          >  2:596005/1‑139 (MQ=255)    ggtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACgg                                                                                                                       <  1:973954/139‑1 (MQ=255)      tcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGt                                                                                                                     >  1:340577/1‑139 (MQ=255)      tcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGt                                                                                                                     >  1:78491/1‑139 (MQ=255)          cAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCt                                                                                                                 >  2:997129/1‑139 (MQ=255)                        gCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCtt                                                                                                   <  1:997129/139‑1 (MQ=255)                                   gATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGc                                                                                        >  1:747800/1‑139 (MQ=255)                                     tCTGACCACTGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGc                                                                                                                  >  1:761910/1‑111 (MQ=255)                                     tCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGc                                                                                                                  <  2:761910/111‑1 (MQ=255)                                                    ttttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATTACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                       <  2:78491/139‑1 (MQ=255)                                                    ttttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                       <  2:340577/139‑1 (MQ=255)                                                        gagaAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaa                                                                          <  1:204148/132‑1 (MQ=255)                                                        gagaAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaa                                                                          >  2:204148/1‑132 (MQ=255)                                                                        ttCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                       <  2:613284/119‑1 (MQ=255)                                                                        ttCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                       >  1:613284/1‑119 (MQ=255)                                                                                   cAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGc                                        >  2:1028261/1‑139 (MQ=255)                                                                                     aaaaGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATc                                                                     >  1:496371/1‑108 (MQ=255)                                                                                     aaaaGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATc                                                                     <  2:496371/108‑1 (MQ=255)                                                                                            cACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAccc                                               >  1:372842/1‑123 (MQ=255)                                                                                            cACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAccc                                               <  2:372842/123‑1 (MQ=255)                                                                                              ccTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGa                               <  2:357867/137‑1 (MQ=255)                                                                                              ccTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGa                               >  1:357867/1‑137 (MQ=255)                                                                                                  gCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTgggg                                                                                                             >  1:387701/1‑55 (MQ=255)                                                                                                  gCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTgggg                                                                                                             <  2:387701/55‑1 (MQ=255)                                                                                                       aGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGggcgg                                                                                                          >  2:8377/1‑53 (MQ=255)                                                                                                       aGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGggcgg                                                                                                          <  1:8377/53‑1 (MQ=255)                                                                                                                      cATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACtggctggc     >  1:842220/1‑139 (MQ=255)                                                                                                                      cATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCACTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATc                                                            <  2:914634/84‑1 (MQ=255)                                                                                                                      cATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCACTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATc                                                            >  1:914634/1‑84 (MQ=255)                                                                                                                       atatTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCa    >  1:107182/1‑139 (MQ=255)                                                                                                                         atTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAgg  <  2:842220/139‑1 (MQ=255)                                                                                                                          ttCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAc                                                 <  1:489381/91‑1 (MQ=255)                                                                                                                          ttCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAc                                                 >  2:489381/1‑91 (MQ=255)                                                                                                                                     |                                                                                                                               TCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGG  >  NC_000913/2826281‑2826539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑