| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,827,351 | G→A | *320* (TAG→TAA) | srlE → | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,827,351 | 0 | G | A | 100.0% | 94.0 / NA | 30 | *320* (TAG→TAA) | srlE | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/17); total (13/17) | |||||||||||
GCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTT > NC_000913/2827228‑2827483 | gCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc < 1:564825/139‑1 (MQ=255) tGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCg < 2:346236/139‑1 (MQ=255) acacGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGAccacca < 2:408618/139‑1 (MQ=255) cTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTg < 2:680109/139‑1 (MQ=255) gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc > 1:701131/1‑139 (MQ=255) aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCGATAAAGGCTGAAACATGAc < 1:358448/84‑1 (MQ=255) aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCGATAAAGGCTGAAACATGAc > 2:358448/1‑84 (MQ=255) ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg > 1:459844/1‑139 (MQ=255) gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATACtca > 2:863057/1‑139 (MQ=255) tCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc < 1:107841/50‑1 (MQ=255) tCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc > 2:107841/1‑50 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttatttat < 1:922937/58‑1 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttatttat > 2:1059460/1‑58 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttatttat > 2:922937/1‑58 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttatttat < 1:1059460/58‑1 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgcg > 2:908384/1‑139 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCa > 2:180463/1‑58 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCa < 1:180463/58‑1 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa < 1:992349/94‑1 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa > 2:992349/1‑94 (MQ=255) cTGGTTTGTCTCCGGTTTTACCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGcc > 2:666601/1‑139 (MQ=255) gTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGc < 2:459844/139‑1 (MQ=255) gTTTGTCTCCGGTTTTATCTAGCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGc < 1:666601/139‑1 (MQ=255) ttGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATACTCATCACCTTTCGTGAAGGCGCGCCTGCgg < 1:863057/139‑1 (MQ=255) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCgg < 1:95081/136‑1 (MQ=255) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCgg > 2:95081/1‑136 (MQ=255) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGAcc < 2:701131/139‑1 (MQ=255) ggTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGaa < 1:908384/139‑1 (MQ=255) tatctatcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTa < 1:233476/139‑1 (MQ=255) tcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCtt > 2:285429/1‑139 (MQ=255) | GCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTT > NC_000913/2827228‑2827483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |