Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,860,416 G→A *219* (TAG→TAA pphB → serine/threonine‑specific protein phosphatase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,4160GA100.0% 176.2 / NA 54*219* (TAG→TAApphBserine/threonine‑specific protein phosphatase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/26);  total (28/26)

AACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCG  >  NC_000913/2860291‑2860539
                                                                                                                             |                                                                                                                           
aaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGctcttc                                                                                                                <  1:241153/139‑5 (MQ=255)
        tGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                       <  1:100434/139‑1 (MQ=255)
                  atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             <  1:932099/139‑1 (MQ=255)
                  atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             <  1:606665/139‑1 (MQ=255)
                   tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                            <  1:1001507/139‑1 (MQ=255)
                    ctttGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGCTTTATATTAATACCGGATCGCCGAACAGCGGGAGGCTGTCATTTTATAAAATACAGTAATCTCATGCTCTTCTCTGAAGCATGCGTAACCCg                                                                                           >  2:932099/2‑139 (MQ=255)
                        ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      >  1:219870/1‑109 (MQ=255)
                        ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      <  2:219870/109‑1 (MQ=255)
                        ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                       <  1:30166/139‑1 (MQ=255)
                        ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                       >  2:304295/1‑139 (MQ=255)
                             tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                  >  2:678871/1‑139 (MQ=255)
                                   gTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                            >  1:1109453/1‑139 (MQ=255)
                                      tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  <  2:931932/114‑1 (MQ=255)
                                      tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  >  1:931932/1‑114 (MQ=255)
                                                   aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                            >  2:850537/1‑139 (MQ=255)
                                                       tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                       >  1:671716/1‑92 (MQ=255)
                                                       tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                       <  2:671716/92‑1 (MQ=255)
                                                       tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                        >  1:627581/1‑139 (MQ=255)
                                                       tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                        >  1:812510/1‑139 (MQ=255)
                                                        cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGc                                                                             <  2:261901/117‑1 (MQ=255)
                                                        cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGc                                                                             >  1:261901/1‑117 (MQ=255)
                                                        cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                       >  2:651900/1‑139 (MQ=255)
                                                        cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                       >  2:472874/1‑139 (MQ=255)
                                                                aGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagag                                               <  1:850537/139‑1 (MQ=255)
                                                                    ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                           <  1:989921/139‑1 (MQ=255)
                                                                         ttGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGc                                      >  1:518785/1‑139 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      >  2:888629/1‑57 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      <  1:888629/57‑1 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             >  1:76427/1‑81 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             <  2:76427/81‑1 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                       <  2:327973/87‑1 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                       >  1:327973/1‑87 (MQ=255)
                                                                            aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                   >  1:628039/1‑139 (MQ=255)
                                                                              aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  <  1:1050629/74‑1 (MQ=255)
                                                                              aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  >  2:1050629/1‑74 (MQ=255)
                                                                                  gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgc                             >  1:122577/1‑139 (MQ=255)
                                                                                   aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCa                                                                                                                        <  2:746232/48‑1 (MQ=255)
                                                                                   aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCa                                                                                                                        >  1:746232/1‑48 (MQ=255)
                                                                                      gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  <  1:524762/66‑1 (MQ=255)
                                                                                      gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                  >  2:524762/1‑66 (MQ=255)
                                                                                      gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                            <  2:143421/88‑1 (MQ=255)
                                                                                      gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                            >  1:143421/1‑88 (MQ=255)
                                                                                       ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             >  2:857123/1‑70 (MQ=255)
                                                                                       ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                             <  1:857123/70‑1 (MQ=255)
                                                                                        cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGa                       >  1:248170/1‑139 (MQ=255)
                                                                                            cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                   <  2:122577/139‑1 (MQ=255)
                                                                                               cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                 >  2:58611/1‑58 (MQ=255)
                                                                                               cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                 <  1:58611/58‑1 (MQ=255)
                                                                                               cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                              >  1:781638/1‑77 (MQ=255)
                                                                                               cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                              <  2:781638/77‑1 (MQ=255)
                                                                                                 ggcggcTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      <  1:1040499/36‑1 (MQ=255)
                                                                                                 ggcggcTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                      >  2:1040499/1‑36 (MQ=255)
                                                                                                            ttttATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAAcc   <  1:651900/139‑1 (MQ=255)
                                                                                                             tttATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcg  <  1:304295/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                           
AACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCG  >  NC_000913/2860291‑2860539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: