Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,902,543	G→A	G216G (GGG→GGA)	ygcE →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,902,543	0	G	A	100.0%	184.4 / NA	57	G216G (GGG→GGA)	ygcE	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/25);  total (32/25)

TTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT  >  NC_000913/2902418‑2902677                                                                                                                              |                                                                                                                                             tttGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTgg                                                                                                                                 >  2:909593/1‑139 (MQ=255)     aTGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGgatga                                                                                                                             >  2:437305/1‑139 (MQ=255)         gCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa                                                                                                                         >  2:656225/1‑139 (MQ=255)           aaaTGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc                                                                                                                       >  1:463356/1‑139 (MQ=255)                    gCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAAt                                                                                                              >  2:185847/1‑139 (MQ=255)                          tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                          <  2:182077/105‑1 (MQ=255)                          tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                          >  1:182077/1‑105 (MQ=255)                          tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcgg                                                                                                                     >  2:443495/1‑126 (MQ=255)                          tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcgg                                                                                                                     <  1:443495/126‑1 (MQ=255)                          tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTctct                                                                                                        >  1:519539/1‑139 (MQ=255)                           ccTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCtt                                                                                                       <  1:656225/139‑1 (MQ=255)                            cTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTg                                                                                                      <  2:463356/139‑1 (MQ=255)                                    tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTAc                                                                                        >  1:859167/1‑139 (MQ=255)                                     atCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACa                                                                                       >  1:180120/1‑139 (MQ=255)                                        acacCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCg                                                                                    <  1:185847/139‑1 (MQ=255)                                          accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa                                                                                                                         >  2:737808/1‑106 (MQ=255)                                          accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa                                                                                                                         <  1:737808/106‑1 (MQ=255)                                           ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                            <  2:1105255/86‑1 (MQ=255)                                           ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                            >  1:1105255/1‑86 (MQ=255)                                           ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCAt                                                                                 <  1:904368/139‑1 (MQ=255)                                             acaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATtg                                                                               >  2:158310/1‑139 (MQ=255)                                              caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGcc                                                                                                                                           <  2:505035/84‑1 (MQ=255)                                              caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGcc                                                                                                                                           >  1:505035/1‑84 (MQ=255)                                              caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATtga                                                                              <  2:180120/139‑1 (MQ=255)                                                 gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                            >  1:1089857/1‑80 (MQ=255)                                                 gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                            <  2:1089857/80‑1 (MQ=255)                                                 gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt                                                                                                                                       <  2:715563/85‑1 (MQ=255)                                                 gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt                                                                                                                                       >  1:715563/1‑85 (MQ=255)                                                      aCTGGCGACACATTTCCCGGCAGGCCTGCCGGTTGTTTGTACCACCAGTGCTAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTCATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAAc                                                                      >  2:784971/1‑139 (MQ=255)                                                                    tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc                                                                                                                       >  1:800972/1‑82 (MQ=255)                                                                    tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc                                                                                                                       <  2:800972/82‑1 (MQ=255)                                                                    tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTg                                                          <  1:1005542/137‑1 (MQ=255)                                                                    tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTg                                                          >  2:1005542/1‑137 (MQ=255)                                                                       cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                     <  1:158310/139‑1 (MQ=255)                                                                            ggACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc                                                <  2:519539/139‑1 (MQ=255)                                                                             gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                     >  1:521173/1‑139 (MQ=255)                                                                             gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             >  2:1129032/1‑125 (MQ=255)                                                                             gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             <  1:1129032/125‑1 (MQ=255)                                                                             gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc                                               >  1:592700/1‑139 (MQ=255)                                                                                 gCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTa                                           <  1:909593/139‑1 (MQ=255)                                                                                              accaccAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             >  2:377577/1‑108 (MQ=255)                                                                                              accaccAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             <  1:377577/108‑1 (MQ=255)                                                                                               ccaccaGTGATAAACAGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTg                                   <  2:521173/139‑1 (MQ=255)                                                                                                 accaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             <  2:183145/105‑1 (MQ=255)                                                                                                 accaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa                                                             >  1:183145/1‑105 (MQ=255)                                                                                                   caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGc                                 >  1:1033132/1‑131 (MQ=255)                                                                                                   caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGc                                 <  2:1033132/131‑1 (MQ=255)                                                                                                       gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                     >  1:558866/1‑139 (MQ=255)                                                                                                       gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                     >  1:1059703/1‑139 (MQ=255)                                                                                                        aTAAACCGGTAGAAGCTCTGGGAGCCGGTTTACTCGATGATGAAACGCCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTCTTGATTAACGGCAAAGCACTGCCTAAAGATCCAGTCGCGCACTGGCCGATTAGCtcttc                    >  2:565714/1‑139 (MQ=255)                                                                                                            aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                     >  1:396162/1‑134 (MQ=255)                                                                                                            aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                     <  2:396162/134‑1 (MQ=255)                                                                                                                gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTa                                                                                         >  1:136914/1‑62 (MQ=255)                                                                                                                gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTa                                                                                         <  2:136914/62‑1 (MQ=255)                                                                                                                gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg                                                       >  2:129984/1‑96 (MQ=255)                                                                                                                gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg                                                       <  1:129984/96‑1 (MQ=255)                                                                                                                        tctGGGAGCCGGATTACTGGATGATGAAACGGCGCTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg    <  1:565714/139‑1 (MQ=255)                                                                                                                          tGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCt  >  2:1041441/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                             TTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT  >  NC_000913/2902418‑2902677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑