Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,979,478	A→G	H153R (CAT→CGT)	lysR →	transcriptional activator of lysA, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,979,478	0	A	G	100.0%	187.7 / NA	52	H153R (CAT→CGT)	lysR	transcriptional activator of lysA, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

CGCAGCTCCTGCAACCCTTTCTGGCACGTTATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACATCAGCCT  >  NC_000913/2979349‑2979610                                                                                                                                  |                                                                                                                                     cGCAGCTCCTGCAACCCTTTCTGGCACGTTATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGc                                                                                                                             <  2:793067/139‑1 (MQ=255)          tGCAACCCTTTCTGGCACGTTATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGa                                                                                                                    <  2:512576/139‑1 (MQ=255)                             ttATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTActctct                                                                                                 >  2:909869/1‑139 (MQ=255)                              tATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCtt                                                                                                <  1:1115700/139‑1 (MQ=255)                              tATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCtt                                                                                                <  2:473141/139‑1 (MQ=255)                                    gATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATg                                                                                          <  2:65350/139‑1 (MQ=255)                                       gTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAg                                                                                       >  1:323310/1‑139 (MQ=255)                                       gTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCATCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGaa                                                                                                                        >  1:485117/1‑106 (MQ=255)                                       gTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCATCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGaa                                                                                                                        <  2:485117/106‑1 (MQ=255)                                          aGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgt                                                                                    <  2:375951/139‑1 (MQ=255)                                           gCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCtt                                                                                                <  1:557319/126‑1 (MQ=255)                                           gCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCtt                                                                                                >  2:557319/1‑126 (MQ=255)                                                      gTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACg                                                                                                                 <  2:370841/98‑1 (MQ=255)                                                      gTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACg                                                                                                                 >  1:370841/1‑98 (MQ=255)                                                      gTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAc                                                                                                      <  2:547847/109‑1 (MQ=255)                                                      gTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAc                                                                                                      >  1:547847/1‑109 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGa                                                                                                                    >  1:933288/1‑94 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGa                                                                                                                    <  2:933288/94‑1 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACg                                                                                                                 <  1:28417/97‑1 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACg                                                                                                                 >  2:28417/1‑97 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAct                                                                                                     <  1:11424/109‑1 (MQ=255)                                                       tGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAct                                                                                                     >  2:11424/1‑109 (MQ=255)                                                         ccccAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTg                                                                                                                              <  2:610509/82‑1 (MQ=255)                                                         ccccAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTg                                                                                                                              >  1:610509/1‑82 (MQ=255)                                                         ccccAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGa                                                                                         >  2:535346/1‑119 (MQ=255)                                                         ccccAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGa                                                                                         <  1:535346/119‑1 (MQ=255)                                                                    aCCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGc                                                                                                                             >  1:427527/1‑72 (MQ=255)                                                                    aCCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGc                                                                                                                             <  2:427527/72‑1 (MQ=255)                                                                    aCCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTa                                                                                                               <  2:842921/86‑1 (MQ=255)                                                                    aCCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTa                                                                                                               >  1:842921/1‑86 (MQ=255)                                                                    aCCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTg                                                          >  2:209513/1‑139 (MQ=255)                                                                         tACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGt                                                     >  2:108043/1‑139 (MQ=255)                                                                             tGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTaaaa                                                 <  2:323310/139‑1 (MQ=255)                                                                             tGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTaaaa                                                 <  1:209513/139‑1 (MQ=255)                                                                                   gTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTa                                                                                                               <  2:203370/71‑1 (MQ=255)                                                                                   gTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTa                                                                                                               >  1:203370/1‑71 (MQ=255)                                                                                    tGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAct                                                                                                     >  2:198774/1‑80 (MQ=255)                                                                                    tGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTAct                                                                                                     <  1:198774/80‑1 (MQ=255)                                                                                     ggCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATg                                                                                          >  2:363155/1‑90 (MQ=255)                                                                                     ggCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATg                                                                                          <  1:363155/90‑1 (MQ=255)                                                                                     ggCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTAtt                                         >  1:977832/1‑139 (MQ=255)                                                                                            ggCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGATCAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCg                                  >  1:184923/1‑139 (MQ=255)                                                                                                 aGCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGgatga                             >  2:572315/1‑139 (MQ=255)                                                                                                  gCGTCATGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGgatgat                            >  1:190364/1‑139 (MQ=255)                                                                                                        tGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGaa                                                                                                                        >  1:615199/1‑41 (MQ=255)                                                                                                        tGATTTAGGACTCACTGAAACGCTCCGTACGCCTGCGGGaa                                                                                                                        <  2:615199/41‑1 (MQ=255)                                                                                                                     aCTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACa         >  1:1055047/1‑139 (MQ=255)                                                                                                                     aCTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACa         >  2:1057177/1‑139 (MQ=255)                                                                                                                     aCTGAAACGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACa         >  1:1021371/1‑139 (MQ=255)                                                                                                                          aaCGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACATCAGc    <  2:977832/139‑1 (MQ=255)                                                                                                                            cGCTCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACATCAGCCt  <  2:190364/139‑1 (MQ=255)                                                                                                                               tCCGTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtg                                                                                     <  1:769439/53‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CGCAGCTCCTGCAACCCTTTCTGGCACGTTATCCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCGGCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACATCAGCCT  >  NC_000913/2979349‑2979610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑