Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,015,738	G→A	*193* (TAG→TAA)	mocA →	CTP:molybdopterin cytidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,015,738	0	G	A	95.5%	61.3 / ‑5.8	22	*193* (TAG→TAA)	mocA	CTP:molybdopterin cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (11/10);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGC  >  NC_000913/3015606‑3015850                                                                                                                                     |                                                                                                                 tGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACg                                                                                                            <  1:1058896/139‑1 (MQ=255)      ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                       >  2:1033597/1‑139 (MQ=255)                                    aTCATTATTCCGTCGAAATGGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                         >  1:755121/1‑139 (MQ=255)                                    aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                         >  1:953514/1‑139 (MQ=255)                                        ttattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGc                                                                     >  1:870687/1‑139 (MQ=255)                                         tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                    <  1:1033597/139‑1 (MQ=255)                                                  gAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATc                                                           <  2:953514/139‑1 (MQ=255)                                                          aaaTGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGAt                                                   <  1:422076/139‑1 (MQ=255)                                                          aaaTGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGAt                                                   <  2:870687/139‑1 (MQ=255)                                                           aaTGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                  <  2:755121/139‑1 (MQ=255)                                                                                atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                  <  1:68984/102‑1 (MQ=255)                                                                                atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                  >  2:68984/1‑102 (MQ=255)                                                                                  tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaa                           >  2:584641/1‑139 (MQ=255)                                                                                       cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTaaa                                                                                                    >  1:613602/1‑61 (MQ=255)                                                                                       cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTaaa                                                                                                    <  2:613602/61‑1 (MQ=255)                                                                                            gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                    >  1:832442/1‑88 (MQ=255)                                                                                            gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                    <  2:832442/88‑1 (MQ=255)                                                                                            gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                 >  2:876870/1‑139 (MQ=255)                                                                                                  tttATTAAAGCGAAGGAACAGTATACGGAAGTTTAGAAAACTTAGTTTTATGTTTTAGTTCAATTATTTAATCATTCCGGCCTTAATTATCATAATGATAAGCAATCACTGATTTTGAGAAAAGAGAAAAATTGATaaa           >  2:759351/1‑139 (MQ=255)                                                                                                           gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGAGTGCGTTCAAATATATTATCCTTCCTGCACTCATTATCATAATGATAAGGAATGACTGATTTTGAGAAAAGAGAAAATTTAAAAAATACGCTGGc  >  2:634725/1‑139 (MQ=255)                                                                                                                           cTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                  >  2:237735/1‑75 (MQ=255)                                                                                                                           cTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                  <  1:237735/75‑1 (MQ=255)                                                                                                                                     |                                                                                                                 TGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGC  >  NC_000913/3015606‑3015850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑