Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,094,234 T→C T283A (ACA→GCA)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,094,2340TC100.0% 159.9 / NA 45T283A (ACA→GCA) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

CGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGC  >  NC_000913/3094098‑3094354
                                                                                                                                        |                                                                                                                        
cGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGgtgcgt                                                                                                                        >  2:421208/1‑139 (MQ=255)
 gTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGtt                                                                                                                       >  2:1038677/1‑139 (MQ=255)
 gTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGtt                                                                                                                       >  1:943096/1‑139 (MQ=255)
      aaaCGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                                  <  1:1038677/139‑1 (MQ=255)
           cccTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCAGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAAt                                                                                                             >  2:811590/1‑139 (MQ=255)
                        cGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCa                                                                                                <  1:1072241/139‑1 (MQ=255)
                           tGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgc                                                                                             >  2:150274/1‑139 (MQ=255)
                                 tAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                       <  1:1018715/139‑1 (MQ=255)
                                     tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCa                                                                                                <  2:996726/126‑1 (MQ=255)
                                     tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCa                                                                                                >  1:996726/1‑126 (MQ=255)
                                     tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTg                                                                                   >  2:629740/1‑139 (MQ=255)
                                           gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                             <  2:33991/139‑1 (MQ=255)
                                           gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                             <  2:862734/139‑1 (MQ=255)
                                               cGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACt                                                                         >  1:887665/1‑139 (MQ=255)
                                                   atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCa                                                                     <  1:421208/139‑1 (MQ=255)
                                                        ccccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCttt                                                                <  1:888068/139‑1 (MQ=255)
                                                            aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTtgtg                                                            >  2:782740/1‑139 (MQ=255)
                                                                   gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                     >  1:513865/1‑139 (MQ=255)
                                                                       ggTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGcc                                                 <  1:811590/139‑1 (MQ=255)
                                                                        gTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGccc                                                >  1:622605/1‑139 (MQ=255)
                                                                                   aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                                     >  2:548642/1‑75 (MQ=255)
                                                                                   aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                                     <  1:548642/75‑1 (MQ=255)
                                                                                     gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                                   <  1:629740/139‑1 (MQ=255)
                                                                                     gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                                   >  2:156769/1‑139 (MQ=255)
                                                                                        gTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAg                                <  2:513865/139‑1 (MQ=255)
                                                                                            cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGa                            >  1:1120792/1‑139 (MQ=255)
                                                                                               gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTGCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTCCCCGTAAACTACCTGCCAGTTGATTa                         >  2:721621/1‑139 (MQ=255)
                                                                                               gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTa                         >  1:838004/1‑139 (MQ=255)
                                                                                                  ggTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGc                      >  2:139937/1‑139 (MQ=255)
                                                                                                   gTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                     >  2:342248/1‑107 (MQ=255)
                                                                                                   gTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                     <  1:342248/107‑1 (MQ=255)
                                                                                                   gTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCCGTTTATCCACTTCCATCTTTTGTGACTGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCa                     <  1:721621/139‑1 (MQ=255)
                                                                                                      ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg                  <  1:150274/139‑1 (MQ=255)
                                                                                                       tCCCTTCGCGAATCAAATTCCCCACCGCGGGGGCGTTAATCAGCAATTCAAATATCGCCACGCGTCCTTCCTGTTTATGCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTCCGCACggg                 >  2:694890/1‑139 (MQ=255)
                                                                                                             cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCaa                                                                                                         >  2:261999/1‑45 (MQ=255)
                                                                                                             cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCaa                                                                                                         <  1:261999/45‑1 (MQ=255)
                                                                                                             cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCttt           >  1:992278/1‑139 (MQ=255)
                                                                                                                      aaTTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTgcgc  <  2:943096/139‑1 (MQ=255)
                                                                                                                       aTTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                           >  1:519532/1‑113 (MQ=255)
                                                                                                                       aTTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                           <  2:519532/113‑1 (MQ=255)
                                                                                                                            ccACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                       <  1:993437/48‑1 (MQ=255)
                                                                                                                            ccACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                       >  2:993437/1‑48 (MQ=255)
                                                                                                                              aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                            >  2:1047301/1‑41 (MQ=255)
                                                                                                                              aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                            <  1:1047301/41‑1 (MQ=255)
                                                                                                                                  cgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                       <  2:430307/42‑1 (MQ=255)
                                                                                                                                  cgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                       >  1:430307/1‑42 (MQ=255)
                                                                                                                                        |                                                                                                                        
CGTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGC  >  NC_000913/3094098‑3094354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: