Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,096,673	G→A	*97* (TAG→TAA)	yggU →	UPF0235 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,096,673	0	G	A	100.0%	41.1 / NA	14	*97* (TAG→TAA)	yggU	UPF0235 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

CTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATC  >  NC_000913/3096545‑3096764                                                                                                                                 |                                                                                            ctcGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATg                                                                                   >  2:997360/1‑139 (MQ=255)  tcGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGc                                                                                  >  2:1090393/1‑139 (MQ=255)                  ggTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAg                                                                            <  1:589708/129‑1 (MQ=255)                  ggTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAg                                                                            >  2:589708/1‑129 (MQ=255)                             gCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATg                                                       <  2:702304/139‑1 (MQ=255)                                                                    aacaaaTTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACt                <  2:97200/139‑1 (MQ=255)                                                                                  aTTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATc  <  1:146954/139‑1 (MQ=255)                                                                                  aTTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATc  <  1:997360/139‑1 (MQ=255)                                                                                   ttAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCt                                                                                      >  1:288665/1‑54 (MQ=255)                                                                                   ttAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCt                                                                                      <  2:288665/54‑1 (MQ=255)                                                                                                                 cggcgTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCaa                                                         >  1:1098433/1‑53 (MQ=255)                                                                                                                 cggcgTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCaa                                                         <  2:1098433/53‑1 (MQ=255)                                                                                                                 cggcgTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCg                                                    >  1:162269/1‑58 (MQ=255)                                                                                                                 cggcgTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCg                                                    <  2:162269/58‑1 (MQ=255)                                                                                                                                 |                                                                                            CTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATC  >  NC_000913/3096545‑3096764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑