Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,131,341	C→T	*356* (TAG→TAA)	yghQ ←	putative inner membrane polysaccharide flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,131,341	0	C	T	100.0%	95.4 / NA	31	*356* (TAG→TAA)	yghQ	putative inner membrane polysaccharide flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/17);  total (14/17)

CGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACA  >  NC_000913/3131220‑3131467                                                                                                                          |                                                                                                                               cGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGc                                                                                                               >  2:218015/1‑139 (MQ=255)  gaagaaACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                              >  2:974424/1‑139 (MQ=255)       aaCGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAGATGACATGACTGAGTCAGCCGAGAAGAATTACCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                         <  2:840338/139‑1 (MQ=255)                     gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                         >  1:183875/1‑125 (MQ=255)                     gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                         <  2:183875/125‑1 (MQ=255)                        gATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAaca                                                                                        >  2:911976/1‑139 (MQ=255)                                   tttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATg                                                                             >  2:1025715/1‑139 (MQ=255)                                     tACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGc                                                                                                               >  1:601682/1‑103 (MQ=255)                                     tACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGc                                                                                                               <  2:601682/103‑1 (MQ=255)                                     tACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGc                                                                                                               <  2:46387/103‑1 (MQ=255)                                     tACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGc                                                                                                               >  1:46387/1‑103 (MQ=255)                                         ggAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCaaa                                                                                          <  2:947111/120‑1 (MQ=255)                                         ggAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCaaa                                                                                          >  1:947111/1‑120 (MQ=255)                                         ggAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGc                                                                       >  1:893825/1‑139 (MQ=255)                                             aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGc                                                                   <  1:1025715/139‑1 (MQ=255)                                               aaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCcg                                                                 <  1:911976/139‑1 (MQ=255)                                                    cGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGACGACGAt                                                            <  2:1067983/139‑1 (MQ=255)                                                       cTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCaa                                                         <  1:974424/139‑1 (MQ=255)                                                                     aatgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGcca                                           <  1:937387/139‑1 (MQ=255)                                                                              aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATAc                                  >  2:303586/1‑139 (MQ=255)                                                                                tGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCg                                >  2:525721/1‑139 (MQ=255)                                                                                  aGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGAt                              >  2:869613/1‑139 (MQ=255)                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACcgcg                                                  >  1:378878/1‑118 (MQ=255)                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACcgcg                                                  <  2:378878/118‑1 (MQ=255)                                                                                      aGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccg                          <  1:303586/139‑1 (MQ=255)                                                                                        ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgcc                        <  1:525721/139‑1 (MQ=255)                                                                                                     ccccGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCg           <  2:110683/139‑1 (MQ=255)                                                                                                     ccccGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCg           <  2:325731/139‑1 (MQ=255)                                                                                                          cctATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGc                                                                   >  2:715671/3‑78 (MQ=255)                                                                                                          cctATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGc                                                                   <  1:715671/76‑1 (MQ=255)                                                                                                              ttCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcaca  <  1:869613/139‑1 (MQ=255)                                                                                                                          |                                                                                                                               CGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACA  >  NC_000913/3131220‑3131467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑