Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,171,879 C→T *111* (TAG→TAA ygiZ ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8790CT100.0% 103.0 / NA 32*111* (TAG→TAAygiZinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCT  >  NC_000913/3171746‑3171992
                                                                                                                                     |                                                                                                                 
tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATa                                                                                                              >  1:420502/1‑139 (MQ=255)
       cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAat                                                                                                       >  2:460531/1‑139 (MQ=255)
            cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATaaa                                                                                                  >  2:315381/1‑139 (MQ=255)
                 aaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTc                                                                                             <  1:973126/139‑1 (MQ=255)
                           catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                   <  2:420502/139‑1 (MQ=255)
                                          tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                    <  1:553133/139‑1 (MQ=255)
                                           gggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                                                                   >  1:737793/1‑139 (MQ=255)
                                             gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                           <  1:1061541/97‑1 (MQ=255)
                                             gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                           >  2:1061541/1‑97 (MQ=255)
                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                <  2:737793/139‑1 (MQ=255)
                                                               aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                               <  2:833112/139‑1 (MQ=255)
                                                               aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                               <  1:460531/139‑1 (MQ=255)
                                                                         aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                              >  2:942859/1‑98 (MQ=255)
                                                                         aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                              <  1:942859/98‑1 (MQ=255)
                                                                                              tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                    >  2:68204/1‑87 (MQ=255)
                                                                                              tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                    <  1:68204/87‑1 (MQ=255)
                                                                                                   aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAGCCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa           >  1:83943/1‑139 (MQ=255)
                                                                                                   aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                       >  2:58934/1‑95 (MQ=255)
                                                                                                   aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                       <  1:58934/95‑1 (MQ=255)
                                                                                                     gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga         >  1:924862/1‑139 (MQ=255)
                                                                                                      agagCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag        <  2:83943/139‑1 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                           <  1:763543/36‑1 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                           >  2:763543/1‑36 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                    <  1:811805/75‑1 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                    >  2:811805/1‑75 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                   <  1:776055/92‑1 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                   >  2:776055/1‑92 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                     <  2:159508/90‑1 (MQ=255)
                                                                                                          ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                     >  1:159508/1‑90 (MQ=255)
                                                                                                            gTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGGGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt  >  1:750166/1‑139 (MQ=255)
                                                                                                                    gACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                     >  2:221895/1‑112 (MQ=255)
                                                                                                                    gACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                     <  1:221895/112‑1 (MQ=255)
                                                                                                                                     |                                                                                                                 
TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCT  >  NC_000913/3171746‑3171992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: