Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,220,190 G→A A233T (GCT→ACT)  patA → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,220,1900GA100.0% 88.5 / NA 28A233T (GCT→ACT) patAputrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

CGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAA  >  NC_000913/3220059‑3220316
                                                                                                                                   |                                                                                                                              
cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         <  2:155338/139‑1 (MQ=255)
       ggCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTc                                                                                                                  >  1:607394/1‑139 (MQ=255)
          gAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                               <  2:1019283/139‑1 (MQ=255)
               cGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAt                                                                                                          <  2:607394/139‑1 (MQ=255)
                            aCCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGt                                                                                             >  1:111490/1‑139 (MQ=255)
                                tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                         >  2:907168/1‑139 (MQ=255)
                                tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                         >  1:321793/1‑139 (MQ=255)
                                                       ccGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTAt                                                                  >  2:433752/1‑139 (MQ=255)
                                                               tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                          <  1:433752/139‑1 (MQ=255)
                                                               tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                          <  1:907168/139‑1 (MQ=255)
                                                                                       tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATcat                                                                                                                   >  2:749767/1‑56 (MQ=255)
                                                                                       tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCt                                                                                                                   <  1:749767/58‑1 (MQ=255)
                                                                                        gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgcc                                                         <  1:793554/115‑1 (MQ=255)
                                                                                        gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgcc                                                         >  2:793554/1‑115 (MQ=255)
                                                                                          gcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCgg                               <  2:343942/139‑1 (MQ=255)
                                                                                            aCGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGgcg                             <  2:920363/139‑1 (MQ=255)
                                                                                                ctctTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                          <  1:19334/106‑1 (MQ=255)
                                                                                                ctctTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                          >  2:19334/1‑106 (MQ=255)
                                                                                                ctctTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACt                         >  2:795769/1‑139 (MQ=255)
                                                                                                     aaCGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACtgatga                    >  1:421373/1‑139 (MQ=255)
                                                                                                      aCGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTgatgat                   <  1:1096196/139‑1 (MQ=255)
                                                                                                          gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                           >  1:546486/1‑79 (MQ=255)
                                                                                                          gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                           <  2:546486/79‑1 (MQ=255)
                                                                                                          gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTc               >  2:65315/1‑139 (MQ=255)
                                                                                                            gCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGa             <  2:111490/139‑1 (MQ=255)
                                                                                                              aaaaaaaCCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATg           <  2:421373/139‑1 (MQ=255)
                                                                                                                       ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACaaa  <  2:321793/139‑1 (MQ=255)
                                                                                                                       ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACaaa  <  1:620905/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
CGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAA  >  NC_000913/3220059‑3220316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: