Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,255,020 G→A *234* (TAG→TAA yhaK → redox‑sensitive bicupin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,255,0200GA100.0% 109.1 / NA 34*234* (TAG→TAAyhaKredox‑sensitive bicupin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

TGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAG  >  NC_000913/3254891‑3255154
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tGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                                               <  1:753412/139‑1 (MQ=255)
        cGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCGCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGgaga                                                                                                                       <  2:388267/139‑1 (MQ=255)
               tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                <  1:89030/139‑1 (MQ=255)
                     gaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                          >  1:925243/1‑123 (MQ=255)
                     gaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                          <  2:925243/123‑1 (MQ=255)
                           ggcgCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                            <  2:366143/115‑1 (MQ=255)
                           ggcgCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                            >  1:366143/1‑115 (MQ=255)
                             cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                            >  1:110658/1‑113 (MQ=255)
                             cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                            <  2:110658/113‑1 (MQ=255)
                                            aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGgaga                                                                                                                       >  2:1022167/1‑103 (MQ=255)
                                            aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGgaga                                                                                                                       <  1:1022167/103‑1 (MQ=255)
                                            aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                    >  1:83102/1‑138 (MQ=255)
                                            aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                    <  2:83102/138‑1 (MQ=255)
                                              ggggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                                           >  1:310173/1‑97 (MQ=255)
                                              ggggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                                           <  2:310173/97‑1 (MQ=255)
                                                    tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                    >  1:432006/1‑130 (MQ=255)
                                                    tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                    <  2:432006/130‑1 (MQ=255)
                                                                             cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTc                                                  >  1:962544/1‑139 (MQ=255)
                                                                             cGCTGATTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                              >  2:1119633/1‑127 (MQ=255)
                                                                             cGCTGATTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                              <  1:1119633/127‑1 (MQ=255)
                                                                                       cGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAattg                                                                                                                                     <  1:838732/46‑1 (MQ=255)
                                                                                       cGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAattg                                                                                                                                     >  2:838732/1‑46 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACa                                                                                                                             <  1:890930/41‑1 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACa                                                                                                                             >  2:890930/1‑41 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                          >  1:87933/1‑44 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                          <  2:87933/44‑1 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGt                                                                               <  2:136165/87‑1 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGt                                                                               >  1:136165/1‑87 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                         <  1:306768/109‑1 (MQ=255)
                                                                                                    cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                         >  2:306768/1‑109 (MQ=255)
                                                                                                        cTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                  >  1:956769/1‑96 (MQ=255)
                                                                                                        cTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                  <  2:956769/96‑1 (MQ=255)
                                                                                                           tGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGgag                                                                                                                        >  1:289078/1‑39 (MQ=255)
                                                                                                           tGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGgag                                                                                                                        <  2:289078/39‑1 (MQ=255)
                                                                                                                        gCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt       >  1:878223/1‑139 (MQ=255)
                                                                                                                             tCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAg  >  2:67164/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                      
TGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAG  >  NC_000913/3254891‑3255154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: