Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,121	C→T	*310* (TAG→TAA)	yhcC ←	putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,121	0	C	T	100.0%	207.0 / NA	63	*310* (TAG→TAA)	yhcC	putative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

ATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGA  >  NC_000913/3352996‑3353253                                                                                                                              |                                                                                                                                     ataCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                         <  2:885550/139‑1 (MQ=255) ataCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                         <  2:552445/139‑1 (MQ=255) ataCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                         <  2:606386/139‑1 (MQ=255)   aCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGt                                                                                                                       >  2:1101273/1‑139 (MQ=255)     tGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAg                                                                                                                     >  2:23668/1‑139 (MQ=255)       cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                                   >  2:1031522/1‑139 (MQ=255)       cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                                   >  2:1082968/1‑139 (MQ=255)            gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                              <  1:1101273/139‑1 (MQ=255)                 cGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTcc                                                                                                         <  2:671723/139‑1 (MQ=255)                   aaTTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCa                                                                                                       <  2:725419/139‑1 (MQ=255)                        gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                                  <  1:118989/139‑1 (MQ=255)                           tCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                               <  2:4571/139‑1 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                                  <  1:230841/125‑1 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGaa                                                                                               <  2:707605/128‑2 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                                    >  1:1089963/1‑139 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                     >  1:451817/1‑138 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                     <  2:451817/138‑1 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAAGGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                               >  1:707605/1‑128 (MQ=255)                                      ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGATATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                                  >  2:230841/1‑125 (MQ=255)                                         cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                                 <  1:1009993/139‑1 (MQ=255)                                                 cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATc                                                                                              <  1:505841/118‑1 (MQ=255)                                                 cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATc                                                                                              >  2:505841/1‑118 (MQ=255)                                                 cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                         <  1:1031522/139‑1 (MQ=255)                                                                   cccAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                       >  1:974701/1‑139 (MQ=255)                                                                      aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGAc                                                      <  1:795891/137‑1 (MQ=255)                                                                      aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGAc                                                      >  2:795891/1‑137 (MQ=255)                                                                      aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCa                                                    >  1:1120165/1‑139 (MQ=255)                                                                      aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAACCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCa                                                    >  2:978267/1‑139 (MQ=255)                                                                        ttCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCAtt                                                  >  2:19290/1‑139 (MQ=255)                                                                           tGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTccc                                               >  2:506516/1‑139 (MQ=255)                                                                                  tACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGAcc                                                     >  1:652095/1‑126 (MQ=255)                                                                                  tACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGAcc                                                     <  2:652095/126‑1 (MQ=255)                                                                                   aCGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGAt                                                                <  1:1065271/114‑1 (MQ=255)                                                                                   aCGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGAt                                                                >  2:1065271/1‑114 (MQ=255)                                                                                     gTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGTGGTAGCCACGGACGTCCCAGCGCCGATCCATGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCg                                       >  1:840733/1‑137 (MQ=255)                                                                                     gTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCg                                       <  2:840733/137‑1 (MQ=255)                                                                                     gTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAt                                     <  2:885969/139‑1 (MQ=255)                                                                                       tataAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcatt                                                                           >  1:493797/1‑99 (MQ=255)                                                                                       tataAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcatt                                                                           <  2:493797/99‑1 (MQ=255)                                                                                        ataAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAtttt                                  <  2:974701/139‑1 (MQ=255)                                                                                           aaTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGc                               >  1:332707/1‑139 (MQ=255)                                                                                             ttGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcac                             <  1:506516/139‑1 (MQ=255)                                                                                             ttGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAACCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcac                             <  1:978267/139‑1 (MQ=255)                                                                                               gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGc                                                           <  2:247364/107‑1 (MQ=255)                                                                                               gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGc                                                           >  1:247364/1‑107 (MQ=255)                                                                                                tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                              <  1:854322/55‑1 (MQ=255)                                                                                                tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                              >  2:854322/1‑55 (MQ=255)                                                                                                tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                         >  2:56621/1‑76 (MQ=255)                                                                                                tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                         <  1:56621/76‑1 (MQ=255)                                                                                                  tgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAg                        >  1:994902/1‑139 (MQ=255)                                                                                                   gagaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                                  >  2:1087434/1‑64 (MQ=255)                                                                                                   gagaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                                  <  1:1087434/64‑1 (MQ=255)                                                                                                    agaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCg                      >  2:585167/1‑139 (MQ=255)                                                                                                           cGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGa                    <  1:33350/134‑1 (MQ=255)                                                                                                           cGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGa                    >  2:33350/1‑134 (MQ=255)                                                                                                                    ccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCgg      >  1:358231/1‑139 (MQ=255)                                                                                                                        aaCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                          <  1:564210/51‑1 (MQ=255)                                                                                                                        aaCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                          >  2:564210/1‑51 (MQ=255)                                                                                                                        aaCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                         <  1:196/116‑1 (MQ=255)                                                                                                                        aaCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                         >  2:196/1‑116 (MQ=255)                                                                                                                        aaCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGa  >  2:49675/1‑139 (MQ=255)                                                                                                                          cGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                         <  2:479243/50‑1 (MQ=255)                                                                                                                          cGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                         >  1:479243/1‑50 (MQ=255)                                                                                                                              |                                                                                                                                     ATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGA  >  NC_000913/3352996‑3353253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑