Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,189	C→T	G26G (GGC→GGT)	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,189	0	C	T	100.0%	191.4 / NA	60	G26G (GGC→GGT)	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/30);  total (30/30)

TTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAC  >  NC_000913/312054‑312313                                                                                                                                        |                                                                                                                             tttttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGaa                                                                                                                           <  1:184103/139‑1 (MQ=255)          tAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                  >  2:141191/1‑139 (MQ=255)          tAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                  <  1:141191/139‑1 (MQ=255)            gTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGa                                                                                                                            >  2:1051931/1‑127 (MQ=255)               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                             <  1:659444/139‑1 (MQ=255)                 aaCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                            <  2:1106099/138‑1 (MQ=255)                 aaCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                            >  1:1106099/1‑138 (MQ=255)                 aaCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCa                                                                                                           >  1:492282/1‑139 (MQ=255)                  aCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                         >  2:960358/1‑124 (MQ=255)                  aCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                         <  1:960358/124‑1 (MQ=255)                      aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTGAGTCTAAAAGAGGGATGATGCCAGCATACAGGGGTGAGGCTGGTCAACAAGTCAACAtt                                                                                                      >  2:515827/1‑139 (MQ=255)                      aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATAAAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                      >  1:1021512/1‑139 (MQ=255)                                 ttATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                            <  2:294118/122‑1 (MQ=255)                                 ttATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                            >  1:294118/1‑122 (MQ=255)                                  tATCATCTTAACCGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGCGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAGCATTAAGATAATGGaa                                                                                          >  1:801399/1‑139 (MQ=255)                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCgg                                                                                   >  2:209118/1‑137 (MQ=255)                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCgg                                                                                   <  1:209118/137‑1 (MQ=255)                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgag                                                                                 >  2:1001682/1‑139 (MQ=255)                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGcag                                                                                 >  2:751600/1‑139 (MQ=255)                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                       >  1:731556/1‑110 (MQ=255)                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGga                                                                                  <  2:43329/131‑1 (MQ=255)                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGga                                                                                  >  1:43329/1‑131 (MQ=255)                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg                                                                          <  1:1001682/139‑1 (MQ=255)                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg                                                                          >  1:656905/1‑139 (MQ=255)                                                  agAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                       <  2:731556/109‑1 (MQ=255)                                                          aaTGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAg                                                                  <  1:515827/139‑1 (MQ=255)                                                              cAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTgg                                                              >  2:909517/1‑139 (MQ=255)                                                                    gcgcCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                              >  1:558816/1‑101 (MQ=255)                                                                    gcgcCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                              <  2:558816/101‑1 (MQ=255)                                                                          cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                  <  1:909517/139‑1 (MQ=255)                                                                          cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                  <  2:492282/139‑1 (MQ=255)                                                                          cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                  <  2:1021512/139‑1 (MQ=255)                                                                                         gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGa                                                                                           >  1:597952/1‑83 (MQ=255)                                                                                         gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGa                                                                                           <  2:597952/83‑1 (MQ=255)                                                                                                        tCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGtt                                                                >  2:952606/1‑95 (MQ=255)                                                                                                        tCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGtt                                                                <  1:952606/95‑1 (MQ=255)                                                                                                           aaaaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                 <  2:656905/139‑1 (MQ=255)                                                                                                                agGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                  <  1:1069566/101‑1 (MQ=255)                                                                                                                agGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                  >  2:1069566/1‑101 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                       >  2:928476/1‑46 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                       <  1:928476/46‑1 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATgg                                                                                            <  1:244810/57‑1 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATgg                                                                                            >  2:244810/1‑57 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               <  2:606505/102‑1 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               <  2:1060093/102‑1 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               >  1:1060093/1‑102 (MQ=255)                                                                                                                  ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               >  1:606505/1‑102 (MQ=255)                                                                                                                     tGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATAGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa       <  1:751600/139‑1 (MQ=255)                                                                                                                       aTGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAaca     >  1:38849/1‑139 (MQ=255)                                                                                                                         gCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCaa                                                        <  2:298329/86‑1 (MQ=255)                                                                                                                         gCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCaa                                                        >  1:298329/1‑86 (MQ=255)                                                                                                                          ccAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac  <  1:1031174/139‑1 (MQ=255)                                                                                                                            aGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAgg                              <  2:195847/109‑1 (MQ=255)                                                                                                                            aGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAgg                              >  1:195847/1‑109 (MQ=255)                                                                                                                              cATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAg                               >  1:1057838/1‑106 (MQ=255)                                                                                                                              cATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAg                               <  2:1057838/106‑1 (MQ=255)                                                                                                                                    ggggTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagaga                                                                              <  2:548216/53‑1 (MQ=255)                                                                                                                                    ggggTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagaga                                                                              >  1:548216/1‑53 (MQ=255)                                                                                                                                    ggggTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               <  2:535537/84‑1 (MQ=255)                                                                                                                                    ggggTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                               >  1:535537/1‑84 (MQ=255)                                                                                                                                        |                                                                                                                             TTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAC  >  NC_000913/312054‑312313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑