Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,145	C→T	A234T (GCC→ACC)	bioH ←	pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,145	0	C	T	100.0%	106.8 / NA	34	A234T (GCC→ACC)	bioH	pimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/19);  total (15/19)

TGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGG  >  NC_000913/3544011‑3544274                                                                                                                                       |                                                                                                                                  tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCt                                                                                                                               <  1:170598/139‑1 (MQ=255)   tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCttt                                                                                                                             >  2:829316/1‑139 (MQ=255)      gaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGc                                                                                                                          <  2:885710/139‑1 (MQ=255)       aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                         <  2:396370/139‑1 (MQ=255)       aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                         <  2:65344/139‑1 (MQ=255)        agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                        <  1:838436/139‑1 (MQ=255)                tGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGatatat                                                                                                                <  1:829316/139‑1 (MQ=255)                 ggCTGCGGAGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTAAACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCACATGGGTCGATTTGGCGAAGATATATg                                                                                                               >  2:207494/1‑139 (MQ=255)                          gAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                      <  2:1100404/139‑1 (MQ=255)                                      ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGtt                                                                                          >  2:909501/1‑139 (MQ=255)                                         tGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTAt                                                                                       >  1:290308/1‑139 (MQ=255)                                           tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCt                                                                                                                               <  1:1028508/97‑1 (MQ=255)                                           tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCt                                                                                                                               >  2:1028508/1‑97 (MQ=255)                                                     tCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAg                                                                                                                      >  1:131697/1‑96 (MQ=255)                                                     tCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAg                                                                                                                      <  2:131697/96‑1 (MQ=255)                                                             cACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTg                                                                   <  2:290308/139‑1 (MQ=255)                                                               cttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                      <  1:287074/100‑1 (MQ=255)                                                               cttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                      >  2:287074/3‑102 (MQ=255)                                                                        tctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAg                                                        <  1:909501/139‑1 (MQ=255)                                                                            cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                        <  2:603103/71‑1 (MQ=255)                                                                            cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                        >  1:603103/1‑71 (MQ=255)                                                                            cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCg                                                    >  2:208630/1‑139 (MQ=255)                                                                              tCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCAt                                                                                >  2:985756/1‑109 (MQ=255)                                                                              tCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCAt                                                                                <  1:985756/109‑1 (MQ=255)                                                                                  cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCgaga                                              >  1:461139/1‑139 (MQ=255)                                                                                   gCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGAt                                             <  1:207494/139‑1 (MQ=255)                                                                                                        tCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACg                        >  2:973863/1‑139 (MQ=255)                                                                                                          ggCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCTGCACCAGACCGTCGAGATAGCCATACAATCGCaaaa                           >  2:1035232/1‑134 (MQ=255)                                                                                                          ggCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCTGCACCAGACCGTCGAGATAGCCATACAATCGCaaaa                           <  1:1035232/134‑1 (MQ=255)                                                                                                           gCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCa                     >  1:904639/1‑139 (MQ=255)                                                                                                            ccGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGAAATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCAt                    >  1:741113/1‑139 (MQ=255)                                                                                                                          aatGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCa      <  1:973863/139‑1 (MQ=255)                                                                                                                              gcgcATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCgg  <  2:904639/139‑1 (MQ=255)                                                                                                                                  aTGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCg                                                    >  2:51658/1‑85 (MQ=255)                                                                                                                                  aTGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCg                                                    <  1:51658/85‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGG  >  NC_000913/3544011‑3544274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑