Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,615,448	A→G	T70A (ACC→GCC)	nikB →	nickel ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,615,448	0	A	G	100.0%	144.5 / NA	40	T70A (ACC→GCC)	nikB	nickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

GCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCTC  >  NC_000913/3615318‑3615582                                                                                                                                   |                                                                                                                                       gctgcGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGACTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTtg                                                                                                                                >  2:719294/1‑139 (MQ=255)          cGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGgcg                                                                                                                       >  2:442604/1‑139 (MQ=255)                               cGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCt                                                                                                               <  1:1129609/126‑1 (MQ=255)                               cGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCt                                                                                                               >  2:1129609/1‑126 (MQ=255)                                          gtctgtctAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgccag                                                                                       >  2:487777/1‑139 (MQ=255)                                                  aaCCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                               <  1:442604/139‑1 (MQ=255)                                                  aaCCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                               <  1:487777/139‑1 (MQ=255)                                                        ccgccgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTAc                                                                         >  1:113961/1‑139 (MQ=255)                                                         cgccgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACt                                                                        >  2:1125104/1‑139 (MQ=255)                                                         cgccgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACt                                                                        <  1:1125104/139‑1 (MQ=255)                                                                            ggCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAgg                                                                                                                         <  2:418323/71‑1 (MQ=255)                                                                            ggCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAgg                                                                                                                         >  1:418323/1‑71 (MQ=255)                                                                                  tACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGccc                                               >  1:950005/1‑139 (MQ=255)                                                                                     ccGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTTGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGcc                                            <  1:719294/139‑1 (MQ=255)                                                                                           cATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTg                                      <  2:113961/139‑1 (MQ=255)                                                                                            aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgccagc                                                                                      >  2:28678/1‑90 (MQ=255)                                                                                            aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgccagc                                                                                      <  1:728954/90‑1 (MQ=255)                                                                                            aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgccagc                                                                                      <  1:28678/90‑1 (MQ=255)                                                                                            aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgccagc                                                                                      >  2:728954/1‑90 (MQ=255)                                                                                             tGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTgg                                     <  2:547813/138‑1 (MQ=255)                                                                                             tGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTgg                                     >  1:547813/1‑138 (MQ=255)                                                                                              gCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACt                                                                                                          >  1:689495/1‑68 (MQ=255)                                                                                              gCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACt                                                                                                          <  2:689495/68‑1 (MQ=255)                                                                                              gCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGaa                                   >  1:850614/1‑139 (MQ=255)                                                                                                       ggATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGt                          >  1:28827/1‑139 (MQ=255)                                                                                                        gATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgc                                                                                          >  1:650621/1‑74 (MQ=255)                                                                                                        gATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgc                                                                                          <  2:650621/74‑1 (MQ=255)                                                                                                        gATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGc                              >  1:506180/1‑134 (MQ=255)                                                                                                        gATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGc                              <  2:506180/134‑1 (MQ=255)                                                                                                         aTCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCAAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCg                                              <  1:592430/117‑1 (MQ=255)                                                                                                         aTCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCAAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCg                                              >  2:592430/1‑117 (MQ=255)                                                                                                             gCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTgcggcg                    <  2:28827/139‑1 (MQ=255)                                                                                                              ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                               <  2:341845/79‑1 (MQ=255)                                                                                                              ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                               >  1:341845/1‑79 (MQ=255)                                                                                                               cGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCt                  <  1:36024/139‑1 (MQ=255)                                                                                                                  tGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAAc                                                        >  1:933905/1‑98 (MQ=255)                                                                                                                  tGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAAc                                                        <  2:933905/98‑1 (MQ=255)                                                                                                                        tCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGc                              <  2:53984/118‑1 (MQ=255)                                                                                                                        tCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGc                              >  1:53984/1‑118 (MQ=255)                                                                                                                               cGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCTc  <  2:950005/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       GCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCTC  >  NC_000913/3615318‑3615582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑