Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,658,893 G→A *176* (TAG→TAA gadE → gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,658,8930GA98.8% 269.9 / NA 83*176* (TAG→TAAgadEgad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/40);  minor base C (1/0);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTA  >  NC_000913/3658780‑3659023
                                                                                                                 |                                                                                                                                  
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                           >  1:574679/1‑139 (MQ=255)
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                           >  1:605881/1‑139 (MQ=255)
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                           >  1:432768/1‑139 (MQ=255)
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                           >  1:424618/1‑139 (MQ=255)
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                           >  1:386562/1‑139 (MQ=255)
    tCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggc                                                                                                       <  2:822693/139‑1 (MQ=255)
        tatTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                   <  2:386562/139‑1 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACt                                                                                                                   <  1:788877/118‑1 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACt                                                                                                                   >  2:788877/1‑118 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              <  2:893749/139‑1 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              >  1:870806/1‑139 (MQ=255)
              gCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg                                                                                             <  2:432768/139‑1 (MQ=255)
                       aTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCa                                                                                    <  2:605881/139‑1 (MQ=255)
                       aTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCa                                                                                    >  1:866473/1‑139 (MQ=255)
                        ttAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAg                                                                                   >  2:457422/1‑139 (MQ=255)
                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                   >  2:242699/1‑115 (MQ=255)
                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                   <  1:242699/115‑1 (MQ=255)
                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                           >  1:983417/1‑139 (MQ=255)
                                           aCATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                                <  2:424618/139‑1 (MQ=255)
                                                aTGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                           <  2:866473/139‑1 (MQ=255)
                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              >  2:142210/1‑103 (MQ=255)
                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              <  1:142210/103‑1 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACt                                                     >  2:665047/1‑139 (MQ=255)
                                                       aGCTGAATCTAAGACGTATGAGCGACATCGTCACCCTCGGTATCACATCTTATATTTACTCAGGACAAAAGCAACTGAAATTGATTGCTGGCATGACGAGGGATGCTGATGCTGATTTCTTTACCTCAGATGATTACTa                                                    >  2:994746/1‑139 (MQ=255)
                                                       aGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa                                                    >  2:689478/1‑139 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                          <  2:1064652/100‑1 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                          >  1:1064652/1‑100 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                <  1:518711/110‑1 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                >  2:518711/1‑110 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                   >  2:472523/1‑139 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              <  1:691564/93‑1 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              >  2:691564/1‑93 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         >  2:982163/1‑130 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         <  1:982163/130‑1 (MQ=255)
                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTCATTTCATTACCCCCGGTGATTACTAAAGga                                               >  1:176118/1‑139 (MQ=255)
                                                             aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGa                                                                                                                              <  1:369497/59‑1 (MQ=255)
                                                             aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGa                                                                                                                              >  2:369497/1‑59 (MQ=255)
                                                                 aaGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGc                                          <  1:665047/139‑1 (MQ=255)
                                                                   gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg                                                                                             <  2:899595/86‑1 (MQ=255)
                                                                   gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg                                                                                             >  1:899595/1‑86 (MQ=255)
                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                           >  1:188210/1‑115 (MQ=255)
                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                           <  2:188210/115‑1 (MQ=255)
                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACg                                   >  1:125787/1‑139 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCg                                                              <  2:317935/111‑1 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCg                                                              >  1:317935/1‑111 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                               >  1:311937/1‑126 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                               <  2:311937/126‑1 (MQ=255)
                                                                              gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                             <  1:472523/139‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTc                                                                        >  1:182848/1‑94 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTc                                                                        <  2:182848/94‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAtt                           <  1:6709/139‑1 (MQ=255)
                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                        >  2:70028/1‑61 (MQ=255)
                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                        <  1:70028/61‑1 (MQ=255)
                                                                                   cGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                        <  1:457422/139‑1 (MQ=255)
                                                                                   cGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTCATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                        <  2:176118/139‑1 (MQ=255)
                                                                                     tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              >  2:147215/1‑67 (MQ=255)
                                                                                     tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                              <  1:147215/67‑1 (MQ=255)
                                                                                         ccTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                  <  2:870806/139‑1 (MQ=255)
                                                                                         ccTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                  <  1:563148/139‑1 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                       >  1:1107547/1‑130 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                       <  2:1107547/130‑1 (MQ=255)
                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAtt                           >  2:42076/1‑121 (MQ=255)
                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAtt                           <  1:42076/121‑1 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGt                                                            <  1:2313/87‑1 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGt                                                            >  2:2313/1‑87 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         <  1:94868/90‑1 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         >  2:94868/1‑90 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                   <  1:712126/44‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                   >  2:712126/1‑44 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         <  1:1064183/86‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                         >  2:1064183/1‑86 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa            <  1:91865/131‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa            >  2:91865/1‑131 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg    >  2:261858/1‑139 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg    >  1:384170/1‑139 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg    >  1:761455/1‑139 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCt                                                                              <  2:905666/63‑1 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCt                                                                              >  1:905666/1‑63 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                             <  1:146832/112‑1 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                             >  2:146832/1‑112 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa  <  2:384170/139‑1 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa  <  2:761455/139‑1 (MQ=255)
                                                                                                         tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa  >  1:382606/1‑139 (MQ=255)
                                                                                                                 |                                                                                                                                  
ACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTA  >  NC_000913/3658780‑3659023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: