Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,768	G→A	*97* (TAG→TAA)	yiaG →	HTH_CROC1 family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,768	0	G	A	100.0%	192.1 / NA	59	*97* (TAG→TAA)	yiaG	HTH_CROC1 family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/25);  total (34/25)

GTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTA  >  NC_000913/3719649‑3719902                                                                                                                        |                                                                                                                                       gTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                                     <  2:491407/139‑1 (MQ=255)        tCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGTAGTTGATGGAATAAATTTTTATCCACTTTATTTCTGTTTa                                                                                                              <  1:692738/139‑1 (MQ=255)        tCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                              <  1:1076319/139‑1 (MQ=255)               ggTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                              >  1:1006432/1‑132 (MQ=255)               ggTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                              <  2:1006432/132‑1 (MQ=255)                         tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                       >  2:56197/1‑129 (MQ=255)                         tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                       <  1:56197/129‑1 (MQ=255)                         tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGa                                                                                             >  1:703958/1‑139 (MQ=255)                          gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                            >  1:304104/1‑139 (MQ=255)                           ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                           >  1:801102/1‑139 (MQ=255)                           ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATCGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                            >  2:541411/1‑138 (MQ=255)                           ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATCGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                            <  1:541411/138‑1 (MQ=255)                               tCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                               <  1:1020631/99‑1 (MQ=255)                               tCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                               >  2:1020631/1‑99 (MQ=255)                                ccAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGtttt                                                                                      >  1:1086397/1‑139 (MQ=255)                                 cAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTc                                                                                     >  1:506381/1‑139 (MQ=255)                                  aGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTcc                                                                                    >  2:903783/1‑139 (MQ=255)                                  aGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTcc                                                                                    <  2:506381/139‑1 (MQ=255)                                     cgcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAc                                                                                 <  1:903783/139‑1 (MQ=255)                                         tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                            <  2:654429/108‑1 (MQ=255)                                         tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                            >  1:654429/1‑108 (MQ=255)                                         tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                              >  1:1059143/1‑138 (MQ=255)                                         tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                              <  2:1059143/138‑1 (MQ=255)                                         tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                             >  1:324504/1‑139 (MQ=255)                                                ttCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                             <  2:289426/132‑1 (MQ=255)                                                ttCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                             >  1:289426/1‑132 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCa                                                                                                                             >  1:290320/1‑76 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCa                                                                                                                             <  2:290320/76‑1 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAg                                                                                               <  2:1131675/106‑1 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAg                                                                                               >  1:1131675/1‑106 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGACATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                               >  2:835605/1‑74 (MQ=255)                                                        ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGACATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                               <  1:835605/74‑1 (MQ=255)                                                                            ttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTAt                                                                                                                       <  2:450182/62‑1 (MQ=255)                                                                            ttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTAt                                                                                                                       >  1:450182/1‑62 (MQ=255)                                                                            ttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAcc                                          <  2:304104/139‑1 (MQ=255)                                                                                         aCCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTgg                             >  1:51151/1‑139 (MQ=255)                                                                                          cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGc                            >  1:997988/1‑139 (MQ=255)                                                                                                aTTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATg                      <  2:997988/139‑1 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCt                                                                                                                   >  2:29761/1‑42 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCt                                                                                                                   <  1:29761/42‑1 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAtt                                                                            <  2:702836/81‑1 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAtt                                                                            >  1:702836/1‑81 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGcaca                  >  2:1008400/1‑139 (MQ=255)                                                                                                    agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGcaca                  >  2:1098875/1‑139 (MQ=255)                                                                                                         gCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAATTATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAAc             >  2:329503/1‑139 (MQ=255)                                                                                                            gTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGt          >  1:403060/1‑139 (MQ=255)                                                                                                             ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                            >  1:875632/1‑56 (MQ=255)                                                                                                             ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                            <  2:875632/56‑1 (MQ=255)                                                                                                             ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt         <  2:1086397/139‑1 (MQ=255)                                                                                                             ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt         >  2:468596/1‑139 (MQ=255)                                                                                                             ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGATCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt         >  2:218390/1‑139 (MQ=255)                                                                                                              tGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGttt        >  2:823115/1‑139 (MQ=255)                                                                                                                aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                             <  1:1029733/68‑1 (MQ=255)                                                                                                                aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                             >  2:1029733/1‑68 (MQ=255)                                                                                                                aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATg                                  >  1:838477/1‑111 (MQ=255)                                                                                                                aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATg                                  <  2:838477/111‑1 (MQ=255)                                                                                                                    aaTAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                              <  2:412540/63‑1 (MQ=255)                                                                                                                    aaTAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                              >  1:412540/1‑63 (MQ=255)                                                                                                                    aaTAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTa  >  2:122176/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                       GTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTA  >  NC_000913/3719649‑3719902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑