Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 153.0 / NA 48P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/22);  total (26/22)

TGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCG  >  NC_000913/323249‑323488
                                                                                                          |                                                                                                                                     
tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                       >  1:243856/1‑139 (MQ=255)
       cGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                                                >  1:849356/1‑139 (MQ=255)
                      tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                 <  1:175253/139‑1 (MQ=255)
                                gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                          >  1:794695/1‑88 (MQ=255)
                                gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                          <  2:794695/88‑1 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                       <  2:828886/121‑1 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                       >  1:828886/1‑121 (MQ=255)
                                        tGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTGCGCCTGCTCtt                                                               >  1:738166/1‑139 (MQ=255)
                                                   ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                         >  2:933036/1‑134 (MQ=255)
                                                   ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                         <  1:933036/134‑1 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGTATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  2:549691/1‑86 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAttt                                                                                                                     <  1:178432/73‑1 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAttt                                                                                                                     >  2:178432/1‑73 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                 >  1:475519/1‑109 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                 <  2:475519/109‑1 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                   <  2:243856/139‑1 (MQ=255)
                                                    tGTCCGGCATATCGACATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  1:549691/86‑1 (MQ=255)
                                                        cGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGGTTGTGa                                               >  1:825170/1‑139 (MQ=255)
                                                             tatCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACg                                          >  1:729002/1‑139 (MQ=255)
                                                                cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                       >  1:892718/1‑75 (MQ=255)
                                                                cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                       <  2:892718/75‑1 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                       >  1:225603/1‑81 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                       <  2:225603/81‑1 (MQ=255)
                                                                               cATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt                                >  1:843780/1‑131 (MQ=255)
                                                                               cATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt                                <  2:843780/131‑1 (MQ=255)
                                                                                 tGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                          >  2:195091/1‑39 (MQ=255)
                                                                                 tGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGt                                                                                                                          <  1:195091/39‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                       <  1:777476/35‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                       >  2:777476/1‑35 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   <  2:457781/87‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   >  1:323093/1‑87 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   <  2:323093/87‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   >  1:457781/1‑87 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa               >  2:376120/1‑139 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa               >  1:639453/1‑139 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATT‑‑TCCGCTACTTGGCGGCTAtaaa                                                                                              <  2:748948/58‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATT‑‑TCCGCTACTTGGCGGCTAtaaa                                                                                              >  1:748948/1‑58 (MQ=255)
                                                                                         gCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                                                    <  2:360965/85‑1 (MQ=255)
                                                                                         gCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                                                    >  1:360965/1‑85 (MQ=255)
                                                                                         gCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                                              >  1:120962/1‑107 (MQ=255)
                                                                                         gCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                                              <  2:120962/107‑1 (MQ=255)
                                                                                          ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  1:846632/48‑1 (MQ=255)
                                                                                          ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  2:846632/1‑48 (MQ=255)
                                                                                                     cAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCg  <  1:376120/139‑1 (MQ=255)
                                                                                                     cAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCg  <  2:729002/139‑1 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                               >  2:43158/1‑44 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                               <  1:43158/44‑1 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                         >  1:328285/1‑82 (MQ=255)
                                                                                                          |                                                                                                                                     
TGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCG  >  NC_000913/323249‑323488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: