Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,819,488 C→T H205H (CAC→CAT
*561* (TAG→TAA
yicG →
ligB ←
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,819,4880CT100.0% 147.5 / NA 46H205H (CAC→CAT
*561* (TAG→TAA
yicG
ligB
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

CATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTC  >  NC_000913/3819353‑3819622
                                                                                                                                       |                                                                                                                                      
cATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                     >  2:363840/1‑139 (MQ=255)
cATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                     <  2:83113/139‑1 (MQ=255)
                    tttCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           >  1:713376/1‑129 (MQ=255)
                    tttCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           <  2:713376/129‑1 (MQ=255)
                               gatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGAACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  1:773364/139‑1 (MQ=255)
                                  gtgGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                                   <  1:363840/139‑1 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  1:181185/139‑1 (MQ=255)
                                              tcCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      >  1:714721/1‑124 (MQ=255)
                                              tcCACCCTGGTATTCGGCTTTTTTGACCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  2:714721/124‑1 (MQ=255)
                                               ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                          >  2:589650/1‑135 (MQ=255)
                                               ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                          <  1:589650/135‑1 (MQ=255)
                                                      ggTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            >  1:701317/1‑126 (MQ=255)
                                                      ggTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:701317/126‑1 (MQ=255)
                                                        tATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTg                                                                             >  1:664713/1‑139 (MQ=255)
                                                          ttCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                           <  2:664713/139‑1 (MQ=255)
                                                           tCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcgc                                                                          >  1:621587/1‑139 (MQ=255)
                                                                           cTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTg                                                                             >  1:450251/1‑120 (MQ=255)
                                                                           cTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTg                                                                             <  2:450251/120‑1 (MQ=255)
                                                                            ttACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                           <  1:437806/89‑1 (MQ=255)
                                                                            ttACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                           >  2:437806/1‑89 (MQ=255)
                                                                                    gcTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGt                                                 <  1:319407/139‑1 (MQ=255)
                                                                                      tACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                           <  2:942365/111‑1 (MQ=255)
                                                                                      tACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                           >  1:942365/1‑111 (MQ=255)
                                                                                        cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTgg                                             <  2:621587/139‑1 (MQ=255)
                                                                                        cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTgg                                             >  2:301443/1‑139 (MQ=255)
                                                                                                gCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTccc                                     <  1:301443/139‑1 (MQ=255)
                                                                                                gCTGGGATTACCGGTCTGTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTccc                                     >  1:708975/1‑139 (MQ=255)
                                                                                                     gATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                                   >  2:155481/1‑72 (MQ=255)
                                                                                                     gATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                                   <  1:155481/72‑1 (MQ=255)
                                                                                                         aCCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTc                            >  2:680883/1‑139 (MQ=255)
                                                                                                              tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:474085/70‑1 (MQ=255)
                                                                                                              tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            >  1:474085/1‑70 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      >  1:176691/1‑50 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  2:176691/50‑1 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAAc                                                                                                >  1:163967/1‑56 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAAc                                                                                                <  2:163967/56‑1 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc             >  2:533620/1‑139 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                                 >  2:642067/1‑53 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                                 <  1:642067/53‑1 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCa           >  1:177096/1‑139 (MQ=255)
                                                                                                                           ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGc                                                                                             >  2:220109/1‑56 (MQ=255)
                                                                                                                           ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGc                                                                                             <  1:220109/56‑1 (MQ=255)
                                                                                                                              cGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                           <  1:757308/71‑1 (MQ=255)
                                                                                                                              cGAAGGACATTAAGGGTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                           >  2:757308/1‑71 (MQ=255)
                                                                                                                                  ggACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCt   <  1:680883/139‑1 (MQ=255)
                                                                                                                                   gACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTc  >  2:959394/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTC  >  NC_000913/3819353‑3819622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: