Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA
G430G (GGC→GGT
cbrA →
dgoT ←
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA100.0% 153.5 / NA 48*355* (TAG→TAA
G430G (GGC→GGT
cbrA
dgoT
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/29);  total (19/29)

TTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870306‑3870576
                                                                                                                                       |                                                                                                                                       
ttACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCa                                                                                                                                      <  2:1096448/139‑1 (MQ=255)
               ccGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCg                                                                                                                       <  2:397850/139‑1 (MQ=255)
                  cAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    <  1:1115864/139‑1 (MQ=255)
                       tGCGTTTAGAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:690590/139‑1 (MQ=255)
                       tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:1130601/139‑1 (MQ=255)
                        gCGTTTAAAACTCTTCGGCAAGATAGTAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:396698/139‑1 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:977752/139‑1 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCACTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:673683/139‑1 (MQ=255)
                          gTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCTATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:712151/136‑1 (MQ=255)
                          gTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  2:712151/1‑136 (MQ=255)
                                             gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGTGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCa                                                                                                                                      >  2:481628/1‑94 (MQ=255)
                                             gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCa                                                                                                                                      <  1:481628/94‑1 (MQ=255)
                                                       aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  2:961149/1‑109 (MQ=255)
                                                       aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:961149/109‑1 (MQ=255)
                                                       aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                                               >  1:1079836/1‑139 (MQ=255)
                                                            aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcg                                                                          >  1:1110071/1‑139 (MQ=255)
                                                             tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:597565/139‑1 (MQ=255)
                                                             tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:1110071/139‑1 (MQ=255)
                                                                       cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                                  <  1:236278/88‑1 (MQ=255)
                                                                       cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                                  >  2:236278/1‑88 (MQ=255)
                                                                                    aaaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCg                                                  <  2:786707/139‑1 (MQ=255)
                                                                                     aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  2:1079836/139‑1 (MQ=255)
                                                                                      aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                         >  1:160842/1‑66 (MQ=255)
                                                                                      aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                         <  2:160842/66‑1 (MQ=255)
                                                                                               tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAAcc                                       >  1:1001179/1‑139 (MQ=255)
                                                                                                 cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCaa                                                                                                                                     >  1:435259/1‑43 (MQ=255)
                                                                                                 cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCaa                                                                                                                                     <  2:435259/43‑1 (MQ=255)
                                                                                                 cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:783117/67‑1 (MQ=255)
                                                                                                 cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  2:783117/1‑67 (MQ=255)
                                                                                                    aGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:780461/62‑1 (MQ=255)
                                                                                                    aGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  2:780461/1‑62 (MQ=255)
                                                                                                     gtggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGCGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  1:596707/122‑1 (MQ=255)
                                                                                                     gtggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGCGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 >  2:596707/1‑122 (MQ=255)
                                                                                                         tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGTGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAgg                             >  2:287252/1‑139 (MQ=255)
                                                                                                           tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:31703/55‑1 (MQ=255)
                                                                                                           tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  1:31703/1‑55 (MQ=255)
                                                                                                                  tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                                >  1:1020715/1‑127 (MQ=255)
                                                                                                                  tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                                <  2:1020715/127‑1 (MQ=255)
                                                                                                                   atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                                            <  1:377613/66‑1 (MQ=255)
                                                                                                                   atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                                            >  2:377613/1‑66 (MQ=255)
                                                                                                                   atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACAAGTAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAATCCTACGCCAGGTACCccacca                   >  2:188377/1‑139 (MQ=255)
                                                                                                                    ttCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGTGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccaccac                  <  1:287252/139‑1 (MQ=255)
                                                                                                                         caGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCaa                                                                                    >  2:833676/1‑68 (MQ=255)
                                                                                                                         caGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCaa                                                                                    <  1:833676/68‑1 (MQ=255)
                                                                                                                          aGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCgg            <  1:630162/139‑1 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGatat                                                                                                       >  2:556477/1‑43 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGatat                                                                                                       <  1:556477/43‑1 (MQ=255)
                                                                                                                                    ttAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAAGTACCCCACCACCAGCGGAACGGTGATg  <  1:188377/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870306‑3870576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: